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Transcription Factor Protocols

Fr. 137.00

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Beschreibung

Produktdetails

Einband

Gebundene Ausgabe

Erscheinungsdatum

15.10.1999

Herausgeber

Martin J. Tymms

Verlag

Humana Press

Seitenzahl

306

Maße (L/B/H)

23.5/16/2.5 cm

Gewicht

644 g

Auflage

2000 edition

Sprache

Englisch

ISBN

978-0-89603-573-7

Beschreibung

Rezension

"Transcription Factor Protocols, volume 130 of Humana Press' popular Methods in Molecular Biology series, aids in the study of transcription factors by providing a compilation of methodologies for the study of the complex interactions between transcription factors, proteins, and DNA. Like the other volumes in this series, the techniques in Transcription Factor Protocols are contributed by an international group of scientists well experienced in the methodologies presented the detailed information provided in Transcription Factor Protocols makes it a welcome addition to the reference collection of an academic library that serves scientists who are conducting molecular biology research. It is well representative of the previous volumes in the Methods in Molecular Biology series"-Cecelia Brown reviewing for E-Streams

"...This book would be a worthwhile addition to the library of any researcher in the general field of transcription regulation...Each of the methods described is accompanied by a well written introductory section setting it in context. The descriptions of the protocols themselves are concisely written in step by step fashion, and readily comprehensible. Recipes for all buffers, with plentiful footnotes describing potential pitfalls, are provided. All chapters end with a detailed bibliography..."-Cell Biology International 2000

"...offers a very complete and up-to-date collection of the techniques needed to explore important aspects in this field...Twenty-two chapters include essential prerequisites, with a clear and extensive coverage of transfection technologies, as well as novel techniques such as the isolation of regulatory DNA sequences by whole genome PCR, functional characterization of such DNA sequences by the use of triplex-forming oligonucleotides, or in vivo footprinting using UV light and ligation-mediated PCR for the identification of protein-bound DNA sequences. The chapters are clearly written by knowledgeable investigators with extensive personal experience of the techniques that the describe. As in previous volumes of the Methods in Molecular Biology series, very detailed step-by-step procedures are provided. Many notes and practical tips are helpful to researchers unfamiliar with the field or new to the most recent and complex strategies." - Cell Biology and Function

"...a book of great importance and which should be in the hands of all cellular and molecular biologists." - Cellular and Molecular Biology

Produktdetails

Einband

Gebundene Ausgabe

Erscheinungsdatum

15.10.1999

Herausgeber

Martin J. Tymms

Verlag

Humana Press

Seitenzahl

306

Maße (L/B/H)

23.5/16/2.5 cm

Gewicht

644 g

Auflage

2000 edition

Sprache

Englisch

ISBN

978-0-89603-573-7

Herstelleradresse

Libri GmbH
Europaallee 1
36244 Bad Hersfeld
DE

Email: gpsr@libri.de

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  • Produktbild: Transcription Factor Protocols
  • Produktbild: Transcription Factor Protocols
  • Isolation of Target Gene Promoter/Enhancer Sequences by Whole Genome PCR Method
    Dennis K. Watson, Richard Kitching, Calvin Vary, Ismail Kola, and Arun Seth

    In Vivo Footprinting Using UV Light and Ligation-Mediated PCR
    Gerd P. Pfeifer and Stella Tommasi

    Identification of DNaseI Hypersensitive Sites Within Nuclei
    Peter N. Cockerill

    Analysis of In Vivo Methylation
    Hans-Henrik M. Dahl and Wendy M. Hutchison

    Detection of Transcription Factor Partners with a Yeast One Hybrid Screen
    Michael Sieweke

    Inverse PCR (IPCR) for Obtaining Promoter Sequence
    Tony Triglia

    PCR-Directed Linker Scanning Mutagenesis
    Kurt Gustin and Robert D. Burk

    Transfection Technologies
    Elaine T. Schenborn

    The Use of Particle-Mediated Gene Transfer for the Study of Promoter Activity in Somatic Tissues
    Charles M. Nicolet and Ning-Sun Yang

    Optimizing Electroporation Conditions for the Transformation of Mammalian Cells
    William C. Heiser

    Calcium Phosphate Transfection of Mammalian Cultured Cells
    Elaine T. Schenborn and Virginia Goiffon

    DEAE-Dextran Transfection of Mammalian Cultured Cells
    Elaine T. Schenborn and Virginia Goiffon

    Liposome-Mediated Transfection of Mammalian Cells
    Elaine T. Schenborn and Jennifer Oler

    Assays for Transcriptional Activity Based on the Luciferase Reporter Gene
    S. Roy Himes and M. Frances Shannon

    Transient Transfection of Schneider Cells in the Study of Transcription Factors
    Guntram Suske

    Triplex-Forming Oligonucleotides and Their Use in the Analysis of Gene Transcription
    Marina Kochetkova and M. Frances Shannon

    Expression and Purification of Histidine-Tagged Transcription Factors
    Donna R. Cohen

    Generation of Transcription Factors in Rabbit Reticulocyte Lysate Depleted of Endogenous DNA-Binding Protein
    Ulrich Kruse, Thorsten T. Ebel, and Albrecht E. Sippel

    Electrophoretic Mobility Shift Assays
    Peter L. Molloy

    In Vitro Promoter Analysis Using Nuclear Extracts and G-Free Cassette Vectors
    Martin J. Tymms

    In Vitro Transcription Using Competitor Oligonucleotides to Deplete Specific Transcription Factors
    Fujiko Watt

    Computer Software of Eukaryotic Promoter Analysis
    Dan S. Prestridge

    Index