Tumour suppressor HIC1 - novel inhibitor of Wnt signalling On generating gene targeted (Hic1 conditional knock-out and Hic1-reporter) mouse strains
-
- Englisch ausgewählt
Fr. 67.90
inkl. gesetzl. MwSt.,
Beschreibung
Produktdetails
Einband
Taschenbuch
Erscheinungsdatum
02.06.2010
Verlag
LAP LAMBERT Academic PublishingSeitenzahl
112
Maße (L/B/H)
22/15/0.7 cm
Gewicht
185 g
Auflage
1. Auflage
Sprache
Englisch
ISBN
978-3-8383-6123-9
Hypermethylated In Cancer 1 (HIC1) gene was identified as a potential tumour suppressor but its physiological role has remained enigmatic. This work (master thesis) provides insights into the biological relevance of HIC1 not only in the context of Wnt signalling. The book contains summary of data on the Wnt signalling pathway and HIC1 available in the literature (current of April 2008) and very broad yet consistent set of laboratory approaches and detailed manuals. These methods can be very helpful to students interested in molecular biology. The whole work is followed by thorough discussion of the presented results. Most interestingly, one chapter of the book describes detailed strategy for generating gene targeted mouse. This is exemplified by production of two strains: (1) a strain for conditional inactivation of Hic1 gene using Cre/loxP system and (2) so-called reporter mouse strain that expresses the fluorescent protein from endogenous Hic1 promoter. A separate file containing the author¿s lecture ''Generation of conditional knock-out mouse step by step'' is included to provide a comprehensive overview of the research method that has pervaded all fields of contemporary biology.
Noch keine Bewertungen vorhanden
Verfassen Sie die erste Bewertung zu diesem Artikel
Helfen Sie anderen Kundinnen und Kunden durch Ihre Meinung.