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Produktbild: Gene Regulation
Band 977

Gene Regulation Methods and Protocols

Aus der Reihe Methods in Molecular Biology

Fr. 138.00

inkl. gesetzl. MwSt., Versandkostenfrei

Beschreibung

Produktdetails

Einband

Gebundene Ausgabe

Erscheinungsdatum

23.02.2013

Abbildungen

XV, 80 illus., 19 illus. in color., farbige Illustrationen, schwarz-weiss Illustrationen

Herausgeber

Minou Bina

Verlag

Humana Press

Seitenzahl

401

Maße (L/B/H)

26/18.3/2.6 cm

Gewicht

912 g

Auflage

2013

Sprache

Englisch

ISBN

978-1-62703-283-4

Beschreibung

Produktdetails

Einband

Gebundene Ausgabe

Erscheinungsdatum

23.02.2013

Abbildungen

XV, 80 illus., 19 illus. in color., farbige Illustrationen, schwarz-weiss Illustrationen

Herausgeber

Minou Bina

Verlag

Humana Press

Seitenzahl

401

Maße (L/B/H)

26/18.3/2.6 cm

Gewicht

912 g

Auflage

2013

Sprache

Englisch

ISBN

978-1-62703-283-4

Herstelleradresse

Springer-Verlag GmbH
Tiergartenstr. 17
69121 Heidelberg
DE

Email: ProductSafety@springernature.com

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  • Produktbild: Gene Regulation
  • 1. Gene Regulation
    Minou Bina
     
    2. Isolation of Nuclei for use in Genome-wide DNase Hypersensitivity Assays to Probe Chromatin Structure
    Guoyu Ling and David J. Waxman
     
    3. DNase I Digestion of Isolated Nulcei for Genome-Wide Mapping of DNase Hypersensitivity Sites in Chromatin
    Guoyu Ling and David J. Waxman
     
    4. Isolation and Analysis of DNA Derived from Nucleosome-Free Regions
    Matthew Murtha, Yatong Wang, Claudio Basilico, and Lisa Dailey
     
    5. Acquisition of High Quality DNA for Massive Parallel Sequencing by in vivo Chromatin Immunoprecipitation
    M. van den Boogaard, L.Y.E. Wong, V.M. Christoffels and P. Barnett
     
    6. Luciferase Assay to Study the Activity of a Cloned Promoter DNA Fragment
    Nina Solberg and Stefan Krauss
     
    7. Promoter Deletion Analysis using a Dual-luciferase Reporter System
    Yong Zhong Xu, Cynthia Kanagaratham, Sylwia Jancik and Danuta Radzioch
     
    8. Application of mRNA Display for in vitro Selection of DNA-binding Transcription Factor Complexes
    Seiji Tateyama and Hiroshi Yanagawa
     
    9. Isolation of Intracellular Protein:DNA Complexes using HaloCHIP; An Antibody Free Alternative to Chromatin Immunoprecipitation
    Danette L. Daniels and Marjeta Urh
     
    10. A Modified Yeast One-Hybrid System for Genome Wide Identification of Transcription Factor Binding Sites
    Kazuyuki Yanai 
    11. Identifying Specific Protein--DNA Interactions using SILAC--based Quantitative Proteomics
    Cornelia G. Spruijt, H. Irem Baymaz and Michiel Vermeulen
     
    12. Electrophoretic Mobility-Shift and Super-Shift Assays for Studies and Characterization of Protein-DNA Complexes
    Elsie I. Parés-Matos
    13. Combination of Native and Denaturing PAGE for the Detection of Protein Binding Regions in Long Fragments of Genomic DNAKristel Kaer and Mart Speek 
    14. Quantitative NanoProteomics Approach for Protein Complex (QNanoPX) using Gold Nanoparticle-based DNA Probe
    Shu-Hui Chen and Mei-Yin Lin
     
    15. Chromatin Assembly and In Vitro Transcription Analyses for Evaluation of Individual Protein Activities in Multicomponent Transcriptional Complexes
    Takayuki Furumatsu and Hiroshi Asahara  
    16. Using FRET to Monitor Protein-induced DNA Bending: The TBP-TATA Complex as a Model System
    Rebecca H. Blair, James A. Goodrich, and Jennifer F. Kugel
     
    17. Promoter Independent Abortive Transcription Assays Unravel Functional Interactions Between TFIIB and RNA Polymerase
    Simone C. Wiesler, Finn Werner and Robert O. J. Weinzierl
     
    18. Fluorescence Cross-correlation Spectroscopy (FCCS) to Observe Dimerization of Transcription Factors in Living Cells
    Hisayo Sadamoto and Hideki Muto
     
    19. Nuclear Recruitment Assay as a Tool to validate Transcription Factor Interactions in Mammalian Cells
    C.J.J. Boogerd, V.M. Christoffels and P. Barnett 
    20. Preparation of Cell Lines for Single-Cell Analysis of Transcriptional Activation Dynamics
    Ilona U. Rafalska-Metcalf and Susan M. Janicki
     
    21. Peptide Microarrays for Profiling of Serine/threonine Kinase Activity of Recombinant Kinases and Lysates of Cells and Tissue Samples
    Riet Hilhorst, Liesbeth Houkes, Monique Mommersteeg, Joyce Musch, Adriënne van den Berg and Rob Ruijtenbeek
     
    22. Immunoaffinity Purification of Protein Complexes from Mammalian Cells
    Chieri Tomomori-Sato, Shigeo Sato, Ronald C. Conaway,
    and Joan W. Conaway
     
    23. Simple and Efficient Identification of Chromatin Modifying Complexes and Characterization of Complex Composition
    Jeong-Heon Lee and David Skalnik
     
    24. Heavy Methyl-SILAC Labeling Coupled with Liquid Chromatography and High-resolution Mass Spectrometry to Study the Dynamics of Site-specific Histone Methylation
    Xing-Jun Cao, Barry M. Zee and Benjamin A. Garcia
     
    25. Analysis of p300 Occupancy at the Early Stage of Stem Cell Differentiation by Chromatin Immunoprecipitation
    Melanie Le May and Qiao Li
     
    26. Mammalian Two-Hybrid Assays for Studies of Interaction of p300 with Transcription Factors
    Daniela B. Mendonça, Gustavo Mendonça, Lyndon F. Cooper
     
    27. Fluorescence Anisotropy Microplate Assay to Investigate the Interaction of Full-length Steroid Receptor Coactivator-1a with Steroid Receptors
    Chen Zhang, Steven K. Nordeen and David J. Shapiro
     
    28. Use of Histone Deacetylase Inhibitors to Examine the Roles of Bromodomain and Histone Acetylation in p300-dependent Gene Expression
    Jihong Chen and Qiao Li
     
    29. Histone Deacetylases Valproic Acid as a Small Molecule Inducer to Direct the Differentiation of Pluripotent Stem Cells 
    Jihong Chen, Natascha Lacroix and Qiao Li
     
    30. Sedimentation and Immunoprecipitation Assays for Analyzing Complexes that Repress Transcription
    Ping Lu, Bruce S. Hostager, Paul B. Rothman and John D. Colgan
     
    31. Methods for Studies of Protein Interactions with Different DNA Methyltransferases
    Jianchang Yang

     
    11. Identifying Specific Protein--DNA Interactions using SILAC--based Quantitative Proteomics
    Cornelia G. Spruijt, H. Irem Baymaz and Michiel Vermeulen
     
    12. Electrophoretic Mobility-Shift and Super-Shift Assays for Studies and Characterization of Protein-DNA Complexes
    Elsie I. Parés-Matos
    13. Combination of Native and Denaturing PAGE for the Detection of Protein Binding Regions in Long Fragments of Genomic DNAKristel Kaer and Mart Speek 
    14. Quantitative NanoProteomics Approach for Protein Complex (QNanoPX) using Gold Nanoparticle-based DNA Probe
    Shu-Hui Chen and Mei-Yin Lin
     
    15. Chromatin Assembly and In Vitro Transcription Analyses for Evaluation of Individual Protein Activities in Multicomponent Transcriptional Complexes
    Takayuki Furumatsu and Hiroshi Asahara  
    16. Using FRET to Monitor Protein-induced DNA Bending: The TBP-TATA Complex as a Model System
    Rebecca H. Blair, James A. Goodrich, and Jennifer F. Kugel
     
    17. Promoter Independent Abortive Transcription Assays Unravel Functional Interactions Between TFIIB and RNA Polymerase
    Simone C. Wiesler, Finn Werner and Robert O. J. Weinzierl
     
    18. Fluorescence Cross-correlation Spectroscopy (FCCS) to Observe Dimerization of Transcription Factors in Living Cells
    Hisayo Sadamoto and Hideki Muto
     
    19. Nuclear Recruitment Assay as a Tool to validate Transcription Factor Interactions in Mammalian Cells
    C.J.J. Boogerd, V.M. Christoffels and P. Barnett 
    20. Preparation of Cell Lines for Single-Cell Analysis of Transcriptional Activation Dynamics
    Ilona U. Rafalska-Metcalf and Susan M. Janicki
     
    21. Peptide Microarrays for Profiling of Serine/threonine Kinase Activity of Recombinant Kinases and Lysates of Cells and Tissue Samples
    Riet Hilhorst, Liesbeth Houkes, Monique Mommersteeg, Joyce Musch, Adriënne van den Berg and Rob Ruijtenbeek
     
    22. Immunoaffinity Purification of Protein Complexes from Mammalian Cells
    Chieri Tomomori-Sato, Shigeo Sato, Ronald C. Conaway,
    and Joan W. Conaway
     
    23. Simple and Efficient Identification of Chromatin Modifying Complexes and Characterization of Complex Composition
    Jeong-Heon Lee and David Skalnik
     
    24. Heavy Methyl-SILAC Labeling Coupled with Liquid Chromatography and High-resolution Mass Spectrometry to Study the Dynamics of Site-specific Histone Methylation
    Xing-Jun Cao, Barry M. Zee and Benjamin A. Garcia
     
    25. Analysis of p300 Occupancy at the Early Stage of Stem Cell Differentiation by Chromatin Immunoprecipitation
    Melanie Le May and Qiao Li
     
    26. Mammalian Two-Hybrid Assays for Studies of Interaction of p300 with Transcription Factors
    Daniela B. Mendonça, Gustavo Mendonça, Lyndon F. Cooper
     
    27. Fluorescence Anisotropy Microplate Assay to Investigate the Interaction of Full-length Steroid Receptor Coactivator-1a with Steroid Receptors
    Chen Zhang, Steven K. Nordeen and David J. Shapiro
     
    28. Use of Histone Deacetylase Inhibitors to Examine the Roles of Bromodomain and Histone Acetylation in p300-dependent Gene Expression
    Jihong Chen and Qiao Li
     
    29. Histone Deacetylases Valproic Acid as a Small Molecule Inducer to Direct the Differentiation of Pluripotent Stem Cells 
    Jihong Chen, Natascha Lacroix and Qiao Li
     
    30. Sedimentation and Immunoprecipitation Assays for Analyzing Complexes that Repress Transcription
    Ping Lu, Bruce S. Hostager, Paul B. Rothman and John D. Colgan
     
    31. Methods for Studies of Protein Interactions with Different DNA Methyltransferases
    Jianchang Yang

     
    16. Using FRET to Monitor Protein-induced DNA Bending: The TBP-TATA Complex as a Model System
    Rebecca H. Blair, James A. Goodrich, and Jennifer F. Kugel
     
    17. Promoter Independent Abortive Transcription Assays Unravel Functional Interactions Between TFIIB and RNA Polymerase
    Simone C. Wiesler, Finn Werner and Robert O. J. Weinzierl
     
    18. Fluorescence Cross-correlation Spectroscopy (FCCS) to Observe Dimerization of Transcription Factors in Living Cells
    Hisayo Sadamoto and Hideki Muto
     
    19. Nuclear Recruitment Assay as a Tool to validate Transcription Factor Interactions in Mammalian Cells
    C.J.J. Boogerd, V.M. Christoffels and P. Barnett 
    20. Preparation of Cell Lines for Single-Cell Analysis of Transcriptional Activation Dynamics
    Ilona U. Rafalska-Metcalf and Susan M. Janicki
     
    21. Peptide Microarrays for Profiling of Serine/threonine Kinase Activity of Recombinant Kinases and Lysates of Cells and Tissue Samples
    Riet Hilhorst, Liesbeth Houkes, Monique Mommersteeg, Joyce Musch, Adriënne van den Berg and Rob Ruijtenbeek
     
    22. Immunoaffinity Purification of Protein Complexes from Mammalian Cells
    Chieri Tomomori-Sato, Shigeo Sato, Ronald C. Conaway,
    and Joan W. Conaway
     
    23. Simple and Efficient Identification of Chromatin Modifying Complexes and Characterization of Complex Composition
    Jeong-Heon Lee and David Skalnik
     
    24. Heavy Methyl-SILAC Labeling Coupled with Liquid Chromatography and High-resolution Mass Spectrometry to Study the Dynamics of Site-specific Histone Methylation
    Xing-Jun Cao, Barry M. Zee and Benjamin A. Garcia
     
    25. Analysis of p300 Occupancy at the Early Stage of Stem Cell Differentiation by Chromatin Immunoprecipitation
    Melanie Le May and Qiao Li
     
    26. Mammalian Two-Hybrid Assays for Studies of Interaction of p300 with Transcription Factors
    Daniela B. Mendonça, Gustavo Mendonça, Lyndon F. Cooper
     
    27. Fluorescence Anisotropy Microplate Assay to Investigate the Interaction of Full-length Steroid Receptor Coactivator-1a with Steroid Receptors
    Chen Zhang, Steven K. Nordeen and David J. Shapiro
     
    28. Use of Histone Deacetylase Inhibitors to Examine the Roles of Bromodomain and Histone Acetylation in p300-dependent Gene Expression
    Jihong Chen and Qiao Li
     
    29. Histone Deacetylases Valproic Acid as a Small Molecule Inducer to Direct the Differentiation of Pluripotent Stem Cells 
    Jihong Chen, Natascha Lacroix and Qiao Li
     
    30. Sedimentation and Immunoprecipitation Assays for Analyzing Complexes that Repress Transcription
    Ping Lu, Bruce S. Hostager, Paul B. Rothman and John D. Colgan
     
    31. Methods for Studies of Protein Interactions with Different DNA Methyltransferases
    Jianchang Yang

     
    20. Preparation of Cell Lines for Single-Cell Analysis of Transcriptional Activation Dynamics
    Ilona U. Rafalska-Metcalf and Susan M. Janicki
     
    21. Peptide Microarrays for Profiling of Serine/threonine Kinase Activity of Recombinant Kinases and Lysates of Cells and Tissue Samples
    Riet Hilhorst, Liesbeth Houkes, Monique Mommersteeg, Joyce Musch, Adriënne van den Berg and Rob Ruijtenbeek
     
    22. Immunoaffinity Purification of Protein Complexes from Mammalian Cells
    Chieri Tomomori-Sato, Shigeo Sato, Ronald C. Conaway,
    and Joan W. Conaway
     
    23. Simple and Efficient Identification of Chromatin Modifying Complexes and Characterization of Complex Composition
    Jeong-Heon Lee and David Skalnik
     
    24. Heavy Methyl-SILAC Labeling Coupled with Liquid Chromatography and High-resolution Mass Spectrometry to Study the Dynamics of Site-specific Histone Methylation
    Xing-Jun Cao, Barry M. Zee and Benjamin A. Garcia
     
    25. Analysis of p300 Occupancy at the Early Stage of Stem Cell Differentiation by Chromatin Immunoprecipitation
    Melanie Le May and Qiao Li
     
    26. Mammalian Two-Hybrid Assays for Studies of Interaction of p300 with Transcription Factors
    Daniela B. Mendonça, Gustavo Mendonça, Lyndon F. Cooper
     
    27. Fluorescence Anisotropy Microplate Assay to Investigate the Interaction of Full-length Steroid Receptor Coactivator-1a with Steroid Receptors
    Chen Zhang, Steven K. Nordeen and David J. Shapiro
     
    28. Use of Histone Deacetylase Inhibitors to Examine the Roles of Bromodomain and Histone Acetylation in p300-dependent Gene Expression
    Jihong Chen and Qiao Li
     
    29. Histone Deacetylases Valproic Acid as a Small Molecule Inducer to Direct the Differentiation of Pluripotent Stem Cells 
    Jihong Chen, Natascha Lacroix and Qiao Li
     
    30. Sedimentation and Immunoprecipitation Assays for Analyzing Complexes that Repress Transcription
    Ping Lu, Bruce S. Hostager, Paul B. Rothman and John D. Colgan
     
    31. Methods for Studies of Protein Interactions with Different DNA Methyltransferases
    Jianchang Yang