Band 34
Applications of Continuous and Steady-State Methods to Root Biology
Aus der Reihe
Developments in Plant and Soil Sciences
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- Hardcover
- Taschenbuch ausgewählt
- eBook
-
Sprache:Englisch
Fr. 191.00
inkl. gesetzl. MwSt.,
Beschreibung
Produktdetails
Einband
Taschenbuch
Erscheinungsdatum
27.09.2011
Herausgeber
John G. Torrey + weitereVerlag
Springer NetherlandSeitenzahl
248
Maße (L/B/H)
23.5/15.5/1.5 cm
Auflage
Softcover reprint of the original 1st ed. 1989
Sprache
Englisch
ISBN
978-94-010-7502-2
This book provides an excellent illustration of the interrelationship between progress in scientific methodology and conceptual advances, and its publica tion should contribute to further advances. It is well known that major advances in understanding often follow the development of new methods. The development of the acetylene reduction assay for nitrogenase activity provides a good example of this interrelationship between theory and methods. Theoretical knowledge led to a search for substrates for nitro genase that could be assayed for more easily than ammonium, the normal product of the enzyme. The discovery of the reduction of acetylene to ethylene by nitrogenase provided the ideal answer to the problem by provid ing a rapid, specific, nondestructive, and inexpensive assay for nitrogenase activity. This assay is now used by almost every laboratory doing research on nitrogen fixation. However, further use and development of the acetylene reduction assay has shown that it can underestimate nitrogenase activity and can even give incorrect relative values under some circumstances. The major problem is that exposure of legume nodules to acetylene can cause a large increase in the resistance to oxygen diffusion into the nodule. This reduced supply of oxygen decreases the rate of nitrogenase activity within a few minutes.
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