Part I. Reviews: Cell Cycle Regulations
1. Cell Cycle-Regulated Transcription: Effectively Using a Genomics Toolbox
Sara L. Bristow, Adam R. Leman, and Steven B. Haase
2. Cell Cycle Regulation by Checkpoints
Kevin J. Barnum and Matthew O’Connell
3. Interplay Between the Cell Cycle and Double-Strand Break Response in Mammalian Cells
Kate Beishline and Jane Azizkhan-Clifford
4. Cell Cycle Regulation by Protein Degradation
Deana M. Koepp
5. Linking Chromosome Duplication to Segregation Via Sister Chromatid Cohesion
Adam R. Leman and Eishi Noguchi
6. The Greatwall-PP2A Axis in Cell Cycle Control
Peng Wang, Marcos Malumbres, and Vincent Archambault
7. Cell Cycle Regulation by the Nutrient-Sensing Mammalian Target of Rapamycin (mTOR) Pathway
Elisabet Cuyas, Bruna Corominas-Faja, Jorge Joven, and Javier A. Menendez
8. The Senescence Arrest Program and the Cell Cycle
Alessandro Bitto, Elizabeth Crowe, Chad Lerner, Claudio Torres, and Christian Sell
9. Oncogenic Ras Pushes (and Pulls) Cell Cycle Progression Through ERK Activation
Paul M. Campbell
10. Cell Cycle Regulation During Viral Infection
Sumedha Bagga and Michael J. Bouchard
11. The Roles of Cohesins in Mitosis, Meiosis, and Human Health and Disease
Amanda S. Brooker and Karen M. Berkowitz
12. Introductory Review of Computational Cell Cycle Modeling
Andres Kriete, Eishi Noguchi, and Christian Sell
Part II. Protocols: Analyzing Cell Cycle Regulations
13. Cell Cycle Synchronization and Flow Cytometry Analysis of Mammalian Cells
Naoko Yoshizawa-Sugata and Hisao Masai
14. Analyzing Transcription Dynamics During the Budding Yeast Cell Cycle
Adam R. Leman, Sara L. Bristow, and Steven B. Haase
15. Analyzing Cell Cycle Checkpoints in Response to Ionizing Radiation in Mammalian Cells
Bin Wang
16. Analyzing DNA Replication Checkpoint in Budding Yeast
Nicole Hustedt and Kenji Shimada
17. Analyzing Cell Cycle-Dependent Degradation and Ubiquitination in Budding Yeast
Dong-Hwan Kim and Deanna M. Koepp
18. Imaging Analysis of Cell Cycle-Dependent Degradation of Cdt1 in Mammalian Cells
Yasushi Shiomi, Naohiro Suenaga, Miyuki Tanaka, Akiyo Hayashi, and Hideo Nishitani
19. PCNA-Dependent Ubiquitination of Cdt1 and p21 in Mammalian Cells
Akiyo Hayashi, Naohiro Suenaga, Yasushi Shiomi, and Hideo Nishitani
20. Analyzing Cdc2/Cdk1 Activation During Stress Response in Schizosaccharomyces pombe
Miguel A. Rodriguez-Gabriel
21. Analyzing Ras-Associated Cell Proliferation Signaling
Matthew C. Stout, Edgar Asiimwe, James R. Birkenstamm, Su Yeon Kim and Paul M. Campbell
22. Telomere Regulation During Cell Cycle in Fission Yeast
Bettina A. Moser, Ya-Ting Chang, and Toru M. Nakamura
23. Detecting Senescence: Methods and Approaches
Elizabeth P. Crowe, Timothy Nacarelli, Alessandro Bitto, Chad Lerner, Christian Sell, and Claudio Torres
Part III. Protocols: Analyzing Cell Cycle Events and Molecules
24. Analyzing RB and E2F During the G1/S Transition
Michael J. Thwaites, Matthew J. Cecchini, and Frederick A. Dick
25. Analyzing G1/S Transcriptional Controls
Steffi Klier, Sarah Farmer, and Robertus A. M. de Bruin
26. Analysis of Replication Timing Using Synchronized Budding Yeast Cultures
Jie Peng, M. K. Raghuraman, and Wenyi Feng
27. Analysis of ssDNA Gaps and DSBs in Genetically Unstable Yeast Cultures
Jie Peng, M. K. Raghuraman, and Wenyi Feng
28. Chromatin Fractionation Analysis of Licensing Factors in Mammalian Cells
Hideo Nishitani, Masayuki Morino, Yusuke Murakami, Takeshi Maeda, and Yasushi Shiomi
29. Imaging Analysis to Determine Chromatin Binding of Licensing Factors MCM2-7 in Mammalian Cells
Mayayuki Morino, Miyuki Tanaka, Yasuhi Shiomi, and Hideo Nishitani
30. Chromatin Immunoprecipitation to Investigate Origin Association of Replication Factors in Mammalian Cells
Adam R. Leman and Eishi Noguchi
31. Live Cell Fluorescence Imaging for Phenotypic Analysis of Mitosis
Sushama Sivakumar, John R Daum, and Gary J. Gorbsky
32. Analyzing Sister Chromatid Cohesion in Mammalian Cells
Katherine M. Feeney, Laura McFarlane-Majeed, and Joanna L. Parish
33. Affinity Purification of Protein Complexes from Drosophila Embryos in Cell Cycle Studies
Zoltan Lipinszki, Peng Wang, Rhys Grant, Catherine Lindon, Nikola S. Dzhindzhev, Pier Paolo D’Avino, Marcin R. Przewloka, David M. Glover, and Vincent Archambault
34. Tracking Histone Variant Nucleosomes Across the Human Cell Cycle Using Biophysical, Biochemical and Cytological Analyses
Marcin P. Walkiewicz, Minh Bui, Delphine Quénet, and Yamini Dalal
