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  • Produktbild: Nuclear Bodies and Noncoding RNAs
  • Produktbild: Nuclear Bodies and Noncoding RNAs
Band 1262

Nuclear Bodies and Noncoding RNAs Methods and Protocols

Aus der Reihe Methods in Molecular Biology

Fr. 73.90

inkl. gesetzl. MwSt., Versandkostenfrei

Beschreibung

Produktdetails

Einband

Gebundene Ausgabe

Erscheinungsdatum

02.01.2015

Abbildungen

XII, 70 illus., 46 illus. in color., schwarz-weiss Illustrationen, farbige Illustrationen

Herausgeber

Shinichi Nakagawa + weitere

Verlag

Springer Us

Seitenzahl

351

Maße (L/B/H)

26/18.3/2.6 cm

Gewicht

881 g

Auflage

2015

Sprache

Englisch

ISBN

978-1-4939-2252-9

Beschreibung

Produktdetails

Einband

Gebundene Ausgabe

Erscheinungsdatum

02.01.2015

Abbildungen

XII, 70 illus., 46 illus. in color., schwarz-weiss Illustrationen, farbige Illustrationen

Herausgeber

Verlag

Springer Us

Seitenzahl

351

Maße (L/B/H)

26/18.3/2.6 cm

Gewicht

881 g

Auflage

2015

Sprache

Englisch

ISBN

978-1-4939-2252-9

Herstelleradresse

Libri GmbH
Europaallee 1
36244 Bad Hersfeld
DE

Email: GPSR Kontakt

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  • Produktbild: Nuclear Bodies and Noncoding RNAs
  • Produktbild: Nuclear Bodies and Noncoding RNAs
  • Part I: Imaging of Noncoding RNAs and Nuclear Bodies
    1. Visualization of lncRNA by Single Molecule Fluorescence in situ Hybridization
          Margaret Dunagin, Moran N. Cabili, John Rinn, and Arjun Raj
    2.      Super-resolution Imaging of Nuclear Bodies by STED Microscopy
    Yasushi Okada and Shinichi Nakagawa
    3.      High-resolution 3D DNA FISH Using Plasmid Probes and Computational Correction of Optical Aberrations to Study Chromatin Structure at the Sub-megabase Scale
    Luca Giorgetti, Tristan Piolot, and Edith Heard
    4.      Time-lapse Imaging of Nuclear Bodies
          Saskia Hutten, Samuel Swift, and Angus I. Lamond
    5.      Visualization of Nucleic Acids with Synthetic Exciton-controlled Fluorescent Oligonucleotide Probes
    Dan Ohtan Wang and Akimitsu Okamoto
    6.      Live CLEM Imaging to Analyze Nuclear Structures at High-resolution
    Tokuko Haraguchi, Hiroko Osakada, and Takako Koujin
    7.      Ultrastructural Analysis of Nuclear Bodies Using Electron Microscopy
    Sylvie Souquere and Gérard Pierron
    8.      Analyses of Nuclear Proteins and Nucleic Acid Structures Using Atomic Force Microscopy
    Jamie L. Gilmore, Aiko Yoshida, Hirohide Takahashi, Katashi Deguchi, Toshiro Kobori, Emilie Louvet, Masahiro Kumeta, Shige H. Yoshimura, and Kunio Takeyasu
    9.      Genome-wide Co-localization Screening of Nuclear Body Components Using a Fluorescently Tagged FLJ cDNA Clone Library
    Tetsuro Hirose and Naoki Goshima
     
    Part II: Biochemical Analysis of Noncoding RNAs and Nuclear Bodies

    10.  Purification of Specific Chromatin Regions Using Oligonucleotides: Capture Hybridization Analysis of RNA Targets (CHART)
    Christopher P. Davis and Jason A. West
    11.  RNA Antisense Purification (RAP) for Mapping RNA Interactions with Chromatin
    Jesse Engreitz, Eric S. Lander, and Mitchell Guttman
    12.  In situ Dissection of RNA Functional Subunits by Domain-specific Chromatin Isolation by RNA Purification (dChIRP)
    Jeffrey J. Quinn and Howard Y. Chang
    13.  Extracting, Enriching and Identifying Nuclear Body Sub-complexes Using Label-based Quantitative Mass Spectrometry
    Archa Fox, Virja Mehta, Severine Boulon, and Laura Trinkle-Mulcahy
    14.  Studying RNA-binding Protein Interactions with Target mRNAs in Eukaryotic Cells: Native Ribonucleoprotein Immunoprecipitation (RIP) Assays
    Joseph A. Cozzitorto, Maya Jimbo, Saswati Chand, Fernando Blanco, Shruti Lal, Melissa Gilbert, Jordan M. Winter, Myriam Gorospe, and Jonathan R. Brody
    15.  Cross-linking and Immunoprecipitation of Nuclear RNA Binding Proteins
          Quan Li, Yuri Uemura, and Yukio Kawahara
    16.  Purifications of Non-coding RNA and Bound Proteins using FLAG Peptide-conjugated Antisense-oligonucleotides
    Shungo Adachi and Tohru Natsume
             
    Part III: Cell and Molecular Biological Approach Towards the Analysis of Noncoding RNAs and Nuclear Bodies

    17.  MMCT-Mediated Chromosome Engineering Technique Applicable to Functional Analysis of lncRNA and Nuclear Dynamics
    Makiko Meguro-Horike and Shin-ichi Horike
    18.  Reconstitution of Nucleocytoplasmic Transport Using Digitonin-Permeabilized Cells
    Shingo Kose, Tomoko Funakoshi, and Naoko Imamoto
    19.  Genome-wide Analysis of Long Noncoding RNA Turnover
    Hidenori Tani, Naoto Imamachi, Rena Mizutani, Katsutoshi Imamura, Yeondae Kwon, Satoru Miyazaki, Sho Maekawa, Yutaka Suzuki, and Nobuyoshi Akimitsu
    20.  Knockdown of Nuclear-retained Long Noncoding RNAs Using Modified DNA Antisense Oligonucleotides
    Xinying Zong, Lulu Huang, Vidisha Tripathi, Raechel Peralta, Susan M. Freier, Shuling Guo, and Kannanganattu V. Prasanth
    21.  siRNA Screening of Nuclear ProteinsYuko Hasegawa and Shinichi Nakagawa