1. Simultaneous Assessment of cAMP Signaling Events in Different Cellular Compartments Using FRET-Based Reporters
Alex Burdyga and Konstantinos Lefkimmiatis
2. Recording Intracellular cAMP Levels with EPAC-Based FRET Sensors by Fluorescence Lifetime Imaging
Marcel Raspe, Jeffrey Klarenbeek, and Kees Jalink
3. A Novel Approach Combining Real-Time Imaging and the Patch-Clamp Technique to Calibrate FRET-Based Reporters for cAMP in Their Cellular Microenvironment
Andreas Koschinski and Manuela Zaccolo
4. Structure-Based, In Silico Approaches for the Development of Novel cAMP FRET Reporters
Matías Machado and Sergio Pantano
5. Automated Image Analysis of FRET Signals for Subcellular cAMP Quantification
Silas J. Leavesley, Arie Nakhmani, Yi Gao, and Thomas C. Rich
6. Channel-Based Reporters for cAMP Detection
Thomas C. Rich, Wenkuan Xin, Silas J. Leavesley, and Mark Taylor
7. Imaging Sub-Plasma Membrane cAMP Dynamics with Fluorescent Translocation Reporters
Anders Tengholm and Olof Idevall-Hagren
8. Adenoviral Transduction of FRET-Based Biosensors for cAMP in Primary Adult Mouse Cardiomyocytes
Oliver Lomas, Marcella Brescia, Ricardo Carnicer, Stefania Monterisi, Nicoletta Surdo, and Manuela Zaccolo
9. Generation of Transgenic Mice Expressing FRET Biosensors
Daniela Hübscher and Viacheslav O. Nikolaev
10. Photoactivatable Adenylyl Cyclase (PACs) as a Tool to Study cAMP Signaling In Vivo: An Overview
Marina Efetova and Martin Schwärzel
11. Selective Disruption of the AKAP Signaling Complexes
Eileen J. Kennedy and John D. Scott
12. Screening for Small Molecule Disruptors of AKAP-PKA Interactions
Carolin Schächterle, Frank Christian, João Miguel Parente Fernandes, Walter Rosenthal, and Enno Klussmann
13. Structure-Based Bacteriophage Screening for AKAP-Selective PKA Regulatory Subunit Variants
Ryan Walker-Gray and Matthew G. Gold
14. A Yeast-Based High-Throughput Screen for Modulators of Phosphodiesterase Activity
Ana Santos de Medeiros and Charles S. Hoffman
15. Separation of PKA and PKG Signaling Nodes by Chemical Proteomics
Eleonora Corradini, Albert J.R. Heck, and Arjen Scholten
16. Development of Computational Models of cAMP Signaling
Susana R. Neves-Zaph and Roy S. Song