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  • Produktbild: Baculovirus and Insect Cell Expression Protocols
  • Produktbild: Baculovirus and Insect Cell Expression Protocols
Band 1350

Baculovirus and Insect Cell Expression Protocols

Aus der Reihe Methods in Molecular Biology

Fr. 242.00

inkl. gesetzl. MwSt., Versandkostenfrei

Beschreibung

Produktdetails

Einband

Gebundene Ausgabe

Erscheinungsdatum

29.01.2016

Abbildungen

XII, 65 illus., 15 illus. in color., farbige Illustrationen, schwarz-weiss Illustrationen

Herausgeber

David W. Murhammer

Verlag

Springer Us

Seitenzahl

508

Maße (L/B/H)

26/18.3/3.4 cm

Gewicht

1094 g

Auflage

3. Auflage

Sprache

Englisch

ISBN

978-1-4939-3042-5

Beschreibung

Produktdetails

Einband

Gebundene Ausgabe

Erscheinungsdatum

29.01.2016

Abbildungen

XII, 65 illus., 15 illus. in color., farbige Illustrationen, schwarz-weiss Illustrationen

Herausgeber

David W. Murhammer

Verlag

Springer Us

Seitenzahl

508

Maße (L/B/H)

26/18.3/3.4 cm

Gewicht

1094 g

Auflage

3. Auflage

Sprache

Englisch

ISBN

978-1-4939-3042-5

Herstelleradresse

Libri GmbH
Europaallee 1
36244 Bad Hersfeld
DE

Email: gpsr@libri.de

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  • Produktbild: Baculovirus and Insect Cell Expression Protocols
  • Produktbild: Baculovirus and Insect Cell Expression Protocols

  • PART I. Introduction

     

    1 Useful Tips, Widely Used Techniques And Quantifying Cell Metabolic Behavior

                            David W. Murhammer

     

     

    PART II. Baculovirus Molecular Biology/Development of Recombinant Baculoviruses

     

    2 Introduction to Baculovirus Molecular Biology

                            Barbara J. Kelly, Linda A. King And Robert D. Possee

     

    3 Baculovirus Transfer Vectors

                            Robert D. Possee And Linda A. King

     

    4 Recombinant Baculovirus Isolation

                            Linda A. King, Richard Hitchman And Robert D. Possee

     

    5 Gene Expression In Mammalian Cells Using Bacmam, A Modified Baculovirus System

                            James A. Fornwald, Quinn Lu, Frederick M. Boyce And Robert S. Ames

     

     

    PART III. INSECT CELL CULTURE

     

    6 Available Lepidopteran Insect Cell Lines

                            Dwight E. Lynn and Robert E. Harrison

     

    7 Lepidopteran Insect Cell Line Isolation from Insect Tissue

                            Dwight E. Lynn

     

    8 Development Of Serum-Free Media For Lepidopteran Insect Cell Lines

                            Leslie C. L. Chan and Steven Reid

     

    9 Routine Maintenance And Storage Of Lepidopteran Insect Cell Lines And Baculoviruses

                            Dwight E. Lynn and Robert L. Harrison

     

     

    PART IV. Protein Production with Recombinant Baculoviruses

     

    10 Small-Scale Production Of Recombinant Proteins Using The Baculovirus Expression Vector System

                            Jian-Ping Yang

     

    11 Recombinant Protein Production In Large Scale Agitated Bioreactors Using The Baculovirus Expression Vector System

                            Christine M. Thompson, Johnny Montes, Marc G. Aucoin and Amine A. Kamen

     

    12 Protein Expression in Insect and Mammalian Cells Using Baculoviruses in Wave Bioreactors

                            Sue H. Kadwell and Laurie K. Overton

     

    13 Protein Production With Recombinant Baculoviruses in Lepidopteran Larvae

                            Elena Kovaleva and David C. Davis

     

    14 Production Of Virus-Like Particles For Vaccination

                            Christine M. Thompson, Marc G. Aucoin and Amine A. Kamen

     

    15 Alternative Strategies for Expressing Multi-Component Protein Complexes in Insect Cells

                            Stephanie Chen

     

     

    PART V. Recombinant Protein Production with Transformed Insect Cells

     

    16 Transforming Lepidopteran Insect Cells for Continuous Recombinant Protein Expression

                            Robert L. Harrison and Donald L. Jarvis

     

    17 Stable Drosophila Cell Lines: An Alternative Approach to Exogenous Protein Expression

                            Marija Backovic and Thomas Krey

     

    18 Transforming Lepidopteran Insect Cells for Improved Protein Processing and Expression

                            Robert L. Harrison and Donald L. Jarvis

     

     

    PART VI Baculovirus Development and Production for Use as Insecticides

     

    19Introduction To The Use Of Baculoviruses As Biological Insecticides

                            Holly J. R. Popham, Tyasning Nusawardani And Bryony C. Bonning

     

    20 Baculovirus Insecticide Production In Insect Larvae

                            Nikolai Van Beek and David C. Davis

     

    21Evaluation Of The Insecticidal Efficacy Of Wild Type And Recombinant Baculoviruses

                            Holly J. R. Popham, Mark R. Ellersieck, Huarong Li and Bryony C. Bonning

     

     

    PART VII. Miscellaneous Techniques and Applications of the Baculovirus/Insect Cell System

     

    22 Evaluating Baculovirus Infection Using Green Fluorescent Protein and Variants

                            Hsuan-Chen Wu, Hyung Joon Cha and William E. Bentley

     

    23 Tubular Bioreactor for Probing Baculovirus Infection and Protein Production

                            Hsuan-Chen Wu, Yu-Chen Hu and William E. Bentley

     

    24Gene Silencing In Insect Cells Using Rnai

                            Hsuan-Chen Wu, John C. March and William E. Bentley

     

    25 Using the Baculovirus/Insect Cell System to Study Apoptosis

                            Nor Chejanovsky

     

    26 Generation of Envelope-Modified Baculoviruses for Gene Delivery into Mammalian Cells

                            Christian Hofmann