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Produktbild: Semaphorin Signaling
Band 1493

Semaphorin Signaling Methods and Protocols

Aus der Reihe Methods in Molecular Biology

Fr. 192.00

inkl. gesetzl. MwSt., Versandkostenfrei

Beschreibung

Produktdetails

Einband

Gebundene Ausgabe

Erscheinungsdatum

28.10.2016

Abbildungen

XV, 96 illus., 54 illus. in color., farbige Illustrationen, schwarz-weiss Illustrationen

Herausgeber

Jonathan R. Terman

Verlag

Springer Us

Seitenzahl

503

Maße (L/B/H)

26/18.3/3.4 cm

Gewicht

1094 g

Auflage

1st ed. 2017

Sprache

Englisch

ISBN

978-1-4939-6446-8

Beschreibung

Produktdetails

Einband

Gebundene Ausgabe

Erscheinungsdatum

28.10.2016

Abbildungen

XV, 96 illus., 54 illus. in color., farbige Illustrationen, schwarz-weiss Illustrationen

Herausgeber

Jonathan R. Terman

Verlag

Springer Us

Seitenzahl

503

Maße (L/B/H)

26/18.3/3.4 cm

Gewicht

1094 g

Auflage

1st ed. 2017

Sprache

Englisch

ISBN

978-1-4939-6446-8

Herstelleradresse

Libri GmbH
Europaallee 1
36244 Bad Hersfeld
DE

Email: GPSR Kontakt

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  • Produktbild: Semaphorin Signaling
  • 1. Semaphorins and their Signaling Mechanisms

    Laura T. Alto and Jonathan R. Terman

     

    Part I: Biochemical Approaches

    2. Isolating Fc-Tagged SEMA4D Recombinant Protein from 293FT Cells

    Joanne Soong and Glynis A. Scott

     

    3. Expression and Purification of Class 7 Semaphorin and its Plexinc1 Receptor Using Baculovirus-Mediated Mammalian Cell Gene Transduction

    Xiaoyan Chen, Po-Han Chen, and Xiaolin He

     

    4. Immunoaffinity Purification of the Glycosylated Extracellular Fragment of Mouse Plexin A2 Produced in a Mammalian Expression System

    Terukazu Nogi, Emiko Mihara, Norihisa Yasui, and Junichi Takagi

     

    5. Plate-Based Assay for Measuring Direct Semaphorin-Neuropilin Interactions

    Matthew W. Parker and Craig W. Vander Kooi

     

    6. Characterizing Plexin Gtpase Interactions Using Gel Filtration, Surface Plasmon Resonance Spectrometry and Isothermal Titration Calorimetry

    Jeannine Muller-Greven, Soonjeung Kim, Prasanta K. Hota, Yufeng Tong, Susmita Borthakur, and Matthias Buck

     

    7. In Vitro Assay for the Rap Gtpase Activating Protein Activity of the Purified Cytoplasmic Domain of Plexin

    Heath G. Pascoe, Yuxiao Wang, and Xuewu Zhang

     

    8. Characterizing F-Actin Disassembly Induced by the Semaphorin Signaling Component Mical

     Jimok Yoon, Ruei-Jiun Hung, and Jonathan R. Terman

     

    9. Characterizing Erbb-2-Mediated Tyrosine Phosphorylation and Activation of Plexins

    Thomas Worzfeld and Jakub M. Swiercz

     

    10. Characterizing PKA-Mediated Phosphorylation of Plexin Using Purified Proteins

     Taehong Yang and Jonathan R. Terman

     

     

    Part II: In Vitro Approaches

    11. Using Heterologous Cos-7 Cells to Identify Semaphorin Signaling Components  Atsuko Sakurai, Colleen L. Doçi, and J. Silvio Gutkind

     

    12. Analysis of Semaphorin-Induced Growth Cone Collapse and Axon Growth Inhibition

    Lionel Meyer, Andrius Kaselis, Saulius Satkauskas, and Dominique Bagnard

     

    13. Using Rotary Shadow Electron Microscopy to Characterize Semaphorin-Mediated Growth Cone Collapse

    Paul C. Bridgman

     

    14. An Electrical Impedance-Based Method for Quantitative Real-Time Analysis of Semaphorin-Elicited Endothelial Cell Collapse

    Chiara Camillo, Noemi Gioelli, Federico Bussolin, and Guido Serini

     

    15. Regulation of Cortical Dendrite Morphology and Spine Organization by Secreted Semaphorins – A Primary Culture Approach

    Sheng-Shiang Peng and Tracy S. Tran

     

    16. Characterizing Semaphorin Signaling Using Isolated Neurons in Culture

    Sophie Chauvet, Erik Mire, and Fanny Mann

     

    17. Performing Axon Orientation Assays With Secreted Semaphorins And Other Guidance Cues

    Céline Delloye-Bourgeois, Frédéric Moret And Valérie Castellani

     

    18. Assays to Examine Transmembrane Semaphorin Function In Vitro

    Rachel Q. Liu, Kristen Browne, and Timothy P. O’Connor

     

    19. Micro-CALI to Study Localized Roles of the Semaphorin Signaling Component CRMP in Axon Growth

    Yoshio Goshima and Kohtaro Takei

     

    20. Visualizing and Characterizing Semaphorin Endocytic Events Using Quantum Dot Conjugated Proteins

    Ioana Carcea and Deanna L. Benson

     

    21. Visualization of Clathrin-Mediated Endocytosis during Semaphorin-Guided Axonal Growth

    Rurika Itofusa, Takuro Tojima, and Hiroyuki Kamiguchi

     

    22. Tracking Differential Endocytosis and Trafficking of Semaphorin Receptor Complexes in Responding Nerve Growth Cones

    Puneet Dang and Andrew J. W. Furley

     

    23. Antibody-Feeding Assay: A Method to Track the Internalization of Neuropilin-1 and Other Cell Surface Receptors

    Sabrina Rizzolio and Luca Tamagnone

     

    24. Photolithography-Based Substrate Microfabrication for Patterning Semaphorin 3A to Study Neuronal Development

    Maya Shelly, Seong-Il Lee, Giulia Suarato, Yizhi Meng, and Sophie Pautot

     

    25. Characterization of Semaphorin 6A-Mediated Effects on Angiogenesis through Regulation of VEGF Signaling

     Hidetaka Ohnuki and Giovanna Tosato

     

    26. Studying the Effects of Semaphorins on Oligodendrocyte Lineage Cells

    Yasir Ahmed Syed Sarah Ali Abdulla, and Mark. R. N. Kotter

     

    27. Characterizing Semaphorin-Mediated Immune Responses Using an Antigen-Presentation Assay

    Satoshi Nojima and Atsushi Kumanogoh

     

    28. Podocyte Shape Regulation by Semaphorin 3A and MICAL-1

    Alda Tufro

     

    Part III: In Vivo Approaches

    29. In Vivo and In Vitro Knockdown Approaches in the Avian Embryo as a Means to Study Semaphorin Signaling

    Karli Montague, Sarah Guthrie, and Ivana Poparic

     

    30. Assaying the Action of Secreted Semaphorins on Vascular Permeability

    Lucas Treps and Julie Gavard

     

    31. Characterization of the Effects of Semaphorin 4D Signaling on Angiogenesis

    Hua Zhou, Ying-Hua Yang, and John R. Basile

     

    32. Characterizing Semaphorin-Mediated Effects on Sensory and Motor Axon Pathfinding and Connectivity during Embryonic Development

    Rosa Eva Huettl and Andrea B. Huber

     

    33. Experimental Approaches for Studying Semaphorin Signals in Tumor Growth and Metastasis in Mouse Models

    Enrico Moiso, Massimo Accardo, and Luca Tamagnone

     

    34. Characterizing Semaphorin Signaling In Vivo Using C. Elegans

    Akira Nukazuka and Shin Takagi