Part I: Review and Overview Chapters
1. Flower Development in Arabidopsis - There is More to it than Learning your ABCs
Nathanael Prunet and Thomas Jack
2. Flower Development in the Asterid Lineage
Barry Causier and Brendan Davies
3. Grass Flower Development
Hiro-Yuki Hirano, Wakana Tanaka, and Taiyo Toriba
4. Flower Diversity and Angiosperm Diversification
Pamela S. Soltis and Douglas E. Soltis
5. Flower Development - Open Questions and Future Directions
Frank Wellmer, John L. Bowman, Brendan Davies, Cristina Ferrándiz, Jennifer C. Fletcher, Robert G. Franks, Emmanuelle Graciet, Veronica Gregis, Toshiro Ito, Thomas P. Jack, Yuling Jiao, Martin M. Kater, Hong Ma, Elliot M. Meyerowitz, Nathanael Prunet, José Luis Riechmann
PART II: Genetic and Phenotypic Analyses
6. Genetic Screens for Floral Mutants in Arabidopsis thaliana: Enhancers and Suppressors
Thanh Theresa Dinh, Elizabeth Luscher, Shaofang Li, Xigang Liu, So Youn Won, and Xuemei Chen
7. Genetic and Phenotypic Analysis of Shoot Apical and Floral Meristem Development
Mona M. Monfared and Jennifer C. Fletcher
8. Genetic and Phenotypic Analyses of Petal Development in Arabidopsis
Judit Szécsi, Barbara Wippermann and Mohammed Bendahmane
9. Cell Biological Analyses of Anther Morphogenesis and Pollen Viability in Arabidopsisand Rice
Fang Chang, Zaibao Zhang, Yue Jin, and Hong Ma
10. Molecular Cell Biology of Male Meiotic Chromosomes and Isolation of Male Meiocytes in Arabidopsis thaliana
Yingxiang Wang, Zhihao Cheng, Pingli Lu, Ljudmilla Timofejeva, Hong Ma
11. Genetic and Phenotypic Analyses of Carpel Development in Arabidopsis
Vicente Balanzà, Patricia Ballester, Monica Colombo, Chloé Fourquin, Irene Martínez-Fernández, and Cristina Ferrándiz
Part III: Microscopy and Histology
12. Microscopic Analysis of Arabidopsis Ovules
Balaji Enugutti and Kay Schneitz
13. Scanning Electron Microscopy Analysis of Floral Development
Robert G. Franks
14. Detection of mRNA Expression Patterns by Non-radioactive in situ Hybridization on Histological Sections of Floral Tissue
Anna Medzihradszky, Kay Schneitz, and Jan U. Lohmann
15. The GUS Reporter System in Flower Development Studies
Janaki S. Mudunkothge and Beth A. Krizek
Part IV: Experimental Systems
16. A Floral Induction System for the Study of Early Arabidopsis Flower Development
Diarmuid Ó’Maoiléidigh and Frank Wellmer
17. Fluorescence Activated Cell Sorting of Shoot Apical Meristem Cell Types
G. Venugopala Reddy
18. Translating Ribosome Affinity Purification (TRAP) for Cell-specific Translation Profiling in Developing Flowers
Ying Wang and Yuling Jiao
19. Laser-assisted Microdissection Applied to Floral Tissues
Samuel E. Wuest and Ueli Grossniklaus
PartV: Molecular Biology, Genomics, and Systems Biology
20. Identification of Arabidopsis Knock-out Lines for Genes of Interest
José Tomás Matus, Thilia Ferrier, and José Luis Riechmann
21. Gene Expression Analysis by Quantitative Real-time PCR for Floral Tissues
Mariana Bustamante, Jian Jin, Oriol Casagran, Tania Nolan, and José Luis Riechmann
22. Misexpression Approaches for the Manipulation of Flower Development
Yifeng Xu, Eng-Seng Gan, and Toshiro Ito
23. Next Generation Sequencing Applied to Flower Development: RNA-Seq
Jun He and Yuling Jiao
24. Next-Generation Sequencing Applied to Flower Development: ChIP-Seq
Emmanuelle Graciet, Diarmuid Seosamh Ó'Maoiléidigh, and Frank Wellmer
25. Live-Imaging of the Arabidopsis Inflorescence MeristemMarcus G. Heisler
and Carolyn Ohno
26. Gene Regulatory Network Models for Floral Organ Determination
Eugenio Azpeitia, José Davila-Velderrain, Carlos Villarreal, and Elena R. Alvarez-Buylla
