Molecular cloning, expression and purification of novel lipase enzyme
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- Englisch ausgewählt
Fr. 68.90
inkl. gesetzl. MwSt.,
Beschreibung
Produktdetails
Einband
Taschenbuch
Erscheinungsdatum
18.10.2016
Verlag
LAP LAMBERT Academic PublishingSeitenzahl
104
Maße (L/B/H)
22/15/0.7 cm
Gewicht
173 g
Sprache
Englisch
ISBN
978-3-659-97084-9
Shewanella putrefaciens is a gram negative bacterium. S.putrefaciens is also a facultative anaerobe with the ability to reduce iron and manganese as the terminal electron acceptor in the electron transport chain in both solid and liquid media. S.putrefaciens is also recognized by its bright pink colour. They are resistant to changeable environmental conditions. Lipases are also found to be widespread among bacteria, based on homology searches in protein databases and bacterial genomes. Lipase genes have been cloned from fungi and bacteria and then have been searched enzyme activity. In this study, we preferred S. putrefaciens ATCC 8071 because its lipase enzyme was not characterized before. Lipase genes in Shewanella putrefaciens were transferred into E. coli with molecular cloning techniques. Also, lipase enzyme was purified and characterized.
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