1. Extracellular Vesicles: A Brief Overview and Its Role in Precision Medicine
Mingyu Shang, John Ji, Chao Song, Bao Jun Gao, Jason Gang Jin, Winston Patrick Kuo, and Hongjun Kang
2. Red Blood Cells: A Source of Extracellular Vesicles
Winston Patrick Kuo, John Tigges, Vasilis Toxavidis, and Ionita Ghiran
Part I: Isolation of Extracellular Vesicles
3. Isolation of Extracelullar Vesicles by Ultracentrifugation
Fatemeh Momen-Heravi
4. Sequential Filtration: A Gentle Method for the Isolation of Functional Extracellular Vesicles
Mitja L. Heinemann and Jody Vykoukal
5. Paper-Based Devices for Isolation of Extracellular Vesicles
Yi-Hsing Hsiao and Chihchen Chen
6. Filter-Based Extracellular Vesicle mRNA Isolation and High-Throughput Gene Expression Analysis
Cindy M. Yamamoto, Taku Murakami, and Shu-Wing Ng
7. Specific and Generic Isolation of Extracellular Vesicles with Magnetic Beads
Ketil W. Pedersen, Bente Kierulf, and Axl Neurauter
Part II: Purification of Extracellular Vesicles
8. Polymer-Based Purification of Extracellular Vesicles
Peter N. Brown and Hang Yin
9. Size Exclusion Chromatography: A Simple and Reliable Method for Exosome Purification
Richard Lobb and Andreas Möller
10. Purification Protocols for Extracellular Vesicles
Rebecca E. Lane, Darren Korbie, Matt Trau, and Michelle M. Hill
Part III: Characterization of Extracellular Vesicles
11. Characterization of Extracellular Vesicles by Surface Plasmon Resonance
Hyungsoon Im, Katherine Yang, Hakho Lee, and Cesar M. Castro
12. Extracellular Vesicle Isolation and Analysis by Western Blotting
Emma J.K. Kowal, Dmitry Ter-Ovanesyan, Aviv Regev, and George M. Church
13. Analysis of Extracellular Vesicles Using Fluorescence Nanoparticle Tracking Analysis
Pauline Carnell-Morris, Dionne Tannetta, Agnieszka Siupa, Patrick Hole, and Rebecca Dragovic
14. Characterization of Extracellular Vesicles by Flow Cytometry
Virginia Camacho, Vasilis Toxavidis, and John C. Tigges
15. Characterization of Extracellular Vesicles by Size-Exclusion High-Performance Liquid Chromatography (HPLC)
Tao Huang and Jiang He
16. Multi-Surface Antigen Staining of Larger Extracellular Vesicles
Veronika Lukacs-Kornek, Henrike Julich, Sabine Katharina Urban, and Miroslaw Kornek
17. Microcapillary Chip-Based Extracellular Vesicle Profiling System
Takanori Akagi and Takanori Ichiki
Part IV: Imaging of Extracellular Vesicles
18. Detection and Characterization of Extracellular Vesicles by Transmission and Cryo-Transmission Electron Microscopy
Petr Cizmar and Yuana Yuana
19. Imaging of Isolated Extracellular Vesicles Using Fluorescence Microscopy
Dmitry Ter-Ovanesyan, Emma J.K. Kowal, Aviv Regev, George M. Church, and Emanuele Cocucci
Part V: Tracking of Extracellular Vesicles
20. In Vivo Tracking of Extracellular Vesicles in Mice Using Fusion Protein Comprising Lactadherin and Gaussia Luciferase
Yuki Takahashi, Makiya Nishikawa, and Yoshinobu Takakura
21. Tracking Extracellular Vesicles Delivery and RNA Translation Using Multiplexed Reporters
Anthony Yan-Tang Wu and Charles Pin-Kuang Lai
Part VI: Downstream Extracellular Vesicle Applications Genomics and Proteomics
22. Extraction and Analysis of Extracellular Vesicle-Associated miRNAs Following Antibody-Based Extracellular Vesicle Capture from Plasma Samples
Davide Zocco and Natasa Zarovni
23. Extracellular Vesicle miRNA Detection Using Molecular Beacons
Won Jong Rhee and Seunga Jeong
24. Rapid Isolation of Extracellular Vesicles from Blood Plasma with Size-Exclusion Chromatography Followed by Mass Spectrometry-Based Proteomic Profiling
Simion Kreimer and Alexander R. Ivanov
25. An Adaptable Polyethylene Glycol-Based Workflow for Proteomic Analysis of Extracellar Vesicles
Stephanie N. Hurwitz and David G. Meckes, Jr.
Part VII: Isolation of Extracellular Vesicles from Biofluids
26. Isolation of Extracellular Vesicles from Ovarian Follicular Fluid: Evaluation of Ultracentrifugation and Commercial Kits
Shlomit Kenigsberg, Clifford C. Librach, and Juliano C. da Silveira
27. Isolation of Extracellular Vesicles in Saliva Using Density Gradient Ultracentrifugation
Kazuya Iwai, Satoshi Yamamoto, Mitsutaka Yoshida, and Kiyotaka Shiba
28. Isolation of Extracellular Vesicles from Breast Milk
Xin Wang
29. An Integrated Double-Filtration Microfluidic Device for Detection of Extracellular Vesicles from Urine for Bladder Cancer Diagnosis
Li-Guo Liang, Ye-Feng Sheng, Sherry Zhou, Fatih Inci, Lanjuan Li, Utkan Demirci, and ShuQi Wang
Part VIII: Extracellular Vesicles Isolated from Cell Culture, Parasites, and Stem Cells
30. Electric Field-Induced Disruption and Releasing Viable Content from Extracellular Vesicles
Chris Wang, Austin Wang, Fang Wei, David T.W. Wong, and Michael Tu
31. Production and Characterization of Extracellular Vesicles in Malaria
Smart Mbagwu, Michael Walch, Luis Filgueira, and Pierre-Yves Mantel
32. Isolation of Extracellular Vesicles from Stem Cells
Zixin Chen, Yongjun Li, Hong Yu, Yan Shen, Chengwei Ju, Genshan Ma, Yutao Liu, Il-man Kim, Neal L. Weintraub, and Yaoliang Tang
Part IX: Extracellular Vesicles in Mouse Models
33. The Use of Peripheral Extracellular Vesicles for Identification of Molecular Biomarkers in a Solid Tumor Mouse Model
Noemí García-Romero, Gorjana Rackov, Cristobal Belda-Iniesta, and Angel Ayuso-Sacido
Part X: Therapeutic Applications of Extracellular Vesicles
34. Therapeutic Applications of Extracellular Vesicles: Perspectives from Newborn Medicine
Gareth R. Willis, Stella Kourembanas, and S. Alex Mitsialis
35. Therapeutic Use of Tumor Cell-Derived Extracellular Vesicles
Jing Liu, Jingwei Ma, Ke Tang, and Bo Huang
