Series Preface…
Preface…
Table of Contents…
Contributing Authors…
1 Building a Two-Photon Microscope is Easy
Spencer LaVere Smith
2 Using Multiphoton Imaging for Targeted Electrophysiological Recording and Live Cell Imaging of Fluorescently Labeled Neurons from Isolated Retinas
Qiang Chen and Wei Wei
3 Two-Photon Neurotransmitter Uncaging for the Study of Dendritic Integration
Alexandra Tran-Van-Minh, Nelson Rebola, Andreas Hoehne, and David A. DiGregorio
4 Multiphoton Glutamate Uncaging to Study Structural and Functional Plasticity of Dendritic Spines
Ivar S. Stein, Travis C. Hill, Won Chan Oh, Laxmi K. Parajuli, and Karen Zito
5 Two-Photon Fluorescence Imaging of Visually Evoked Glutamate Release using iGluSnFR in the Mouse Visual System
Bart G. Borghuis
6 Imaging Neuronal Signal Transduction using Multiphoton FRET-FLIM
Paul R. Evans, Long Yan, and Ryohei Yasuda
7 Combining Multiphoton Excitation Microscopy with Fast Microiontophoresis to Investigate Neuronal Signaling
Espen Hartveit and Margaret Lin Veruki
8 Multiphoton Excitation Microscopy for Reconstruction and Analysis of Single Neuron Morphology
Espen Hartveit, Bas-Jan Zandt, and Margaret Lin Veruki
9 Serial Multiphoton Tomography and Analysis of Volumetric Images of the Mouse Brain
Denise M.O. Ramirez, Apoorva D. Ajay, Mark P. Goldberg, and Julian P. Meeks
10 Studying a Light Sensor with Light: Multiphoton Imaging in the Retina
Thomas Euler, Katrin Franke, and Tom Baden
11 Multiphoton Ca2+ Imaging of Astrocytes with Genetically Encoded Indicators Delivered by a Viral Approach
Rune Enger, Rolf Sprengel, Erlend A. Nagelhus, and Wannan Tang
12 Two-Photon Imaging of Dendritic Calcium Dynamics In Vivo
Lucy M. Palmer
13 Voltage Imaging with ANNINE Dyes and Two-Photon Microscopy
Christopher J. Roome and Bernd Kuhn
14 High-Speed, Random-Access Multiphoton Microscopy for Monitoring Synaptic and Neuronal Activity in 3D in Behaving Animals
Mate Marosi, Gergely Szalay, Gergely Katona, and Balázs Rózsa