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Produktbild: Two-Hybrid Systems
Band 1794

Two-Hybrid Systems Methods and Protocols

Aus der Reihe Methods in Molecular Biology

Fr. 206.00

inkl. gesetzl. MwSt., Versandkostenfrei

Beschreibung

Produktdetails

Einband

Taschenbuch

Erscheinungsdatum

25.07.2019

Abbildungen

XII, 58 illus., 50 illus. in color., schwarz-weiss Illustrationen, farbige Illustrationen

Herausgeber

Luis Oñate-Sánchez

Verlag

Springer Us

Seitenzahl

355

Maße (L/B/H)

25.4/17.8/1.9 cm

Gewicht

854 g

Auflage

Softcover reprint of the original 1st ed. 2018

Sprache

Englisch

ISBN

978-1-4939-9310-9

Beschreibung

Produktdetails

Einband

Taschenbuch

Erscheinungsdatum

25.07.2019

Abbildungen

XII, 58 illus., 50 illus. in color., schwarz-weiss Illustrationen, farbige Illustrationen

Herausgeber

Luis Oñate-Sánchez

Verlag

Springer Us

Seitenzahl

355

Maße (L/B/H)

25.4/17.8/1.9 cm

Gewicht

854 g

Auflage

Softcover reprint of the original 1st ed. 2018

Sprache

Englisch

ISBN

978-1-4939-9310-9

Herstelleradresse

Libri GmbH
Europaallee 1
36244 Bad Hersfeld
DE

Email: gpsr@libri.de

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  • Produktbild: Two-Hybrid Systems

  • 1. Protein Interactomics by Two-Hybrid Methods

                Soon Gang Choi, Aaron Richardson, Luke Lambourne, David E. Hill, and Marc Vidal

     

    Part I: Yeast Hybrid Systems to Study Protein, Nucleic Acid, and Ligand Interactions

     

    2. Making the Right Choice: Critical Parameters of the Y2H Systems

                Jitender Mehla, J. Harry Caufield, and Peter Uetz

     

    3. Identification of Protein-Protein Interactions Using Pool-Array-Based Yeast Two-Hybrid Screening

                Thomas Lathouwers, Jeroen Wagemans, and Rob Lavigne

     

    4. Detecting Interactions of Membrane Proteins: The Split-Ubiquitin System

                Lisa Yasmin Asseck and Christopher Grefen

     

    5. The Ras Recruitment System (RRS) for the Identification and Characterization of Protein-Protein Interactions

                Ami Aronheim

     

    6. A Bacterial Adenylate Cyclase-Based Two-Hybrid System Compatible with Gateway® Cloning

                Macy G. Olson, Megan Goldammer, Emilie Gauliard, Daniel Ladant, and Scot P. Ouellette

     

    7. Analyses of Protein Interaction Networks Using Computational Tools

                Shaowei Dong and Nicholas J. Provart

     

    8. Options and Considerations When Using a Yeast One-Hybrid System

                Jared A. Sewell and Juan I. Fuxman Bass

     

    9. Screening Arrayed Libraries with DNA and Protein Baits to Identify Interacting Proteins

                Rocío Sánchez-Montesino and Luis Oñate-Sánchez

     

    10. Construction of Arabidopsis Transcription Factor ORFeome Collections and Identification of Protein-DNA Interactions by High-Throughput Yeast One-Hybrid Screens

                S. Earl Kang, Ghislain Breton, and Jose L. Pruneda-Paz

     

    11. Transcription Factor-Centered Yeast One-Hybrid Assay

                Xiaoyu Ji, Liuqiang Wang, Dandan Zang, and Yucheng Wang

     

    12. The Yeast Three-Hybrid System for Protein Interactions

                Franziska Glass and Mizuki Takenaka

     

    13. The Yeast Three-Hybrid System for Screening RNA-Binding Proteins in Plants

                Sung Ki Cho and David J. Hannapel

     

    14. Using Yeast Hybrid System to Identify Proteins Binding to Small Molecules

                You Wang, David S. Letham, Peter C.L. John, and Ren Zhang

     

    15. Shuttling of Entire Libraries from an Entry Vector to a Destination Vector of the Gateway System

                Alexander Heyl

     

    Part II: Experimental Approaches to Study Protein-Protein and Protein-DNA Interactions in Plants and Human Cells

     

    16. Bimolecular Fluorescence Complementation with Improved Gateway-Compatible Vectors to Visualize Protein-Protein Interactions in Plant Cells

                Shino Goto-Yamada, Kazumi Hikino, Mikio Nishimura, Tsuyoshi Nakagawa, and Shoji Mano

     

    17. Bimolecular Fluorescence Complementation to Visualize Protein-Protein Interactions in Human Cells Based on Gateway Cloning Technology

                Adriana Lepur and Oliver Vugrek

     

    18. KISS: A Mammalian Two-Hybrid Method for In Situ Analysis of Protein-Protein Interactions

                Delphine Masschaele, Sarah Gerlo, Irma Lemmens, Sam Lievens, and Jan Tavernier

     

    19. Coimmunoprecipitation of Interacting Proteins in Plants

                Alfonso Muñoz and M. Mar Castellano

     

    20. Analysis of Protein-Protein Interaction by Co-IP in Human Cells

                Zhenyuan Tang and Yoshinori Takahashi

     

    21. Tandem Affinity Purification of Protein Complexes from Arabidopsis Cell Cultures

                Marta García-León, Elisa Iniesto, and Vicente Rubio

     

    22. Transient Transactivation Studies in Nicotiana benthamiana Leaves

                Pilar Lasierra and Salomé Prat

     

    23. The Use of the Chromatin Immunoprecipitation Technique for In Vivo Identification of Plant Protein-DNA Interactions

                José A. Jarillo, Dorota N. Komar, and Manuel Piñeiro

     

    24. Chromatin Immunoprecipitation for Identification of Protein-DNA Interactions in Human Cells

                Bjørk Ditlev Larsen, Martin Rønn Madsen, Ronni Nielsen, and Susanne Mandrup