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  • Produktbild: Essential Guide to Reading Biomedical Papers

Essential Guide to Reading Biomedical Papers

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Beschreibung

Produktdetails

Einband

Taschenbuch

Erscheinungsdatum

23.11.2012

Herausgeber

Philip D. Langton

Verlag

John Wiley & Sons Inc

Seitenzahl

372

Maße (L/B/H)

24.4/17/2 cm

Gewicht

593 g

Auflage

2. Auflage

Sprache

Englisch

ISBN

978-1-119-95997-7

Beschreibung

Rezension

"The book would be of most benefit to new researchers or early career scientists though with some benefits to the more experienced scientist moving into a new discipline." (Microbiology Today, 1 September 2013)
 
"As a guide to how to properly ask questions of life and how to interpret its often-enigmatic answers, this book is a must read. Summing Up: Highly recommended. Upper-division undergraduates, graduate students, researchers/faculty, and professionals." (Choice, 1 August 2013)
 
"Too many recent bioscience graduates lack significant research lab experience. Even research projects can expose them to just a few techniques. Appraisal of research papers in undergraduate courses tends to address the results, but rarely includes a critical evaluation of the researchers methodology. In reality, few bioscientists can claim a working knowledge of more than a handful of the techniques covered in this collection.
 
These shortcomings can be greatly overcome thanks to this book. The contributors are active research scientists of high quality. Each addresses the methods in a critical sense and provide an expert's view of the advantages and pitfalls. There is no equivalent book currently available." Dr David J. Miller on behalf of The Physiological Society
 
"Research questions require the scientist to employ molecular, cell, organism and population approaches, and the reader of scientific literature to carefully consume data from each of those environments. This book provides a clear and concise summary of the research techniques and approaches that generate data. Importantly, the authors identify appropriate uses and cautionary caveats, essential for readers who do not use the technique. This book is essential for accurate and careful interpretation of scientific literature." Professor Robert G. Carroll, Editor Advances in Physiology Education

Produktdetails

Einband

Taschenbuch

Erscheinungsdatum

23.11.2012

Herausgeber

Philip D. Langton

Verlag

John Wiley & Sons Inc

Seitenzahl

372

Maße (L/B/H)

24.4/17/2 cm

Gewicht

593 g

Auflage

2. Auflage

Sprache

Englisch

ISBN

978-1-119-95997-7

Herstelleradresse

Libri GmbH
Europaallee 1
36244 Bad Hersfeld
DE

Email: GPSR Kontakt

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  • Produktbild: Essential Guide to Reading Biomedical Papers
  • Produktbild: Essential Guide to Reading Biomedical Papers
  • List of contributors ix
     
    Foreword xi
     
    Preface xiii
     
    Acknowledgements xvii
     
    Introduction xix
     
    Section A - Basic principles 1
     
    1 Philosophy of science 3
    James Ladyman
     
    2 Ingredients of experimental design 9
    Nick Colegrave
     
    3 Statistics: a journey that needs a guide 17
    Gordon Drummond
     
    Section B - Cell and molecular 27
     
    4 Organ bath pharmacology 29
    Emma Robinson
     
    5 Small vessel myography 39
    Tomoko Kamishima and John M Quayle
     
    6 Mammalian cell cultures: the example of airway epithelial cell cultures for cystic fibrosis research 49
    Scott H Randell
     
    7 Electron microscopy (TEM and SEM) 59
    Paul Verkade
     
    8 Fluorescence microscopy 67
    Mark Jepson
     
    9 Intracellular 'sharp' microelectrode recording 77
    Helena C Parkington and Harold A Coleman
     
    10 Single electrode voltage-clamp (SEVC) 85
    Harold A Coleman and Helena C Parkington
     
    11 Patch clamp recording 95
    Neil Bannister and Phil Langton
     
    12 Production of antibodies 105
    Elek Molnar
     
    13 Immunocytochemistry and immunohistochemistry 117
    Elek Molnar
     
    14 Immunoprecipitation (IP) 129
    David Bates
     
    15 Immunoblotting (western) 137
    Samantha F. Moore, Joshua S. Savage and Ingeborg Hers
     
    16 Applications of green fluorescent protein (GFP) 147
    Mark Jepson
     
    17 Fluorescent measurement of ion activity in cells 153
    Helen Kennedy
     
    18 Detection of exocytosis - real time 163
    Anja Teschemacher
     
    19 Viral vector transgenesis 173
    Anja Teschemacher
     
    20 Polymerase chain reaction (PCR) and reverse transcription (RT)-PCR 179
    Lucy F. Donaldson
     
    21 In situ hybridisation (ISH) 187
    Lucy F. Donaldson
     
    22 Methods of interference (antisense, siRNAs and dominant negative mutations) 193
    Allison Fulford
     
    23 Transcriptome analysis: microarrays 203
    Charles Hindmarch
     
    24 Experimental proteomics 215
    Thierry Le Bihan
     
    Section C - In vivo / integrative 229
     
    25 Behavioural methodology 231
    Emma Robinson
     
    26 Genetically modified mouse models 241
    Nina Balthasar
     
    27 Wireless recording of cardiovascular signals 247
    Julian FR Paton and Fiona D McBryde
     
    28 Electrical stimulation methods 253
    Jon Wakerley
     
    29 Extracellular recording 261
    Jon Wakerley
     
    30 Antidromic identification 271
    Jon Wakerley
     
    31 Event-triggered averaging, including spike-triggered averaging 279
    Richard Apps
     
    32 Axonal transport tracing of CNS pathways 285
    John Crabtree
     
    33 Cardiovascular methods: general considerations for human studies 291
    Erica A Wehrwein and Michael J Joyner
     
    34 Measuring cardiac output in humans 299
    Erica A Wehrwein and Michael J Joyner
     
    35 Measuring peripheral blood flow in humans 311
    Erica A Wehrwein and Michael J Joyner
     
    Index 319