Krishnan, K: AUG start codon recognition by bacterial riboso Ribosome-mRNA interactions that contribute to recognition and binding of a 5 -terminal AUG start codon
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- Englisch ausgewählt
Fr. 75.90
inkl. gesetzl. MwSt.,
Beschreibung
Produktdetails
Einband
Taschenbuch
Erscheinungsdatum
07.05.2014
Verlag
SpsSeitenzahl
148
Maße (L/B/H)
22/15/0.9 cm
Gewicht
237 g
Sprache
Englisch
ISBN
978-3-639-51189-5
Initiation is the rate-limiting step of translation and is an important regulatory step in gene expression. Translation initiation requires ribosome recognition of the mRNA's start codon followed by formation of a stable ternary complex involving initiator tRNA. Studies have shown specific contacts between a AUG start codon and several ribosomal proteins (r-proteins). We employed short-leadered mRNAs in order to further characterize the path of an mRNA's 5'-AUG from initial association with the ribosome to its final placement at the ribosome's decoding site (P-site). Our results suggest that bacterial ribosomes can bind and inspect the 5'-terminus of all mRNAs for an AUG triplet, with close proximity of AUG to the 5'-terminus positively influencing ribosome binding and ternary initiation complex formation. Crosslinking studies showed that while 5'-AUG interactions with r-proteins S1, S3, S4, S7, S10 and S18 can occur in the absence of tRNA-fMet, movement of a 5'-AUG into the Psite requires tRNA-fMet.
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