1. A Method to Predict the 3D Structure of an RNA Scaffold
Xiaojun Xu and Shi-Jie Chen
2. Post-crystallization Improvement of RNA Crystal Diffraction Quality
Jinwei Zhang and Adrian R. Ferré-D’Amaré
3. Expression and Purification of RNA-protein Complexes in Escherichia coli
El Khouri M, Catala M, Seijo B, Chabal J, Tisné C, Dardel F, and Ponchon L
4. Production of Homogeneous Recombinant RNA using a tRNA Scaffold and Hammerhead Ribozymes
Frank H.T. Nelissen, Hans A. Heus, and Sybren S. Wijmenga
5. In vivo Production of Small Recombinant RNAs Embedded in 5S rRNA-Derived Protective Scaffold
Victor G. Stepanov and George E. Fox
6. Detection of RNA-Protein Interactions using Tethered RNA Affinity Capture
IIOKA H. and Macara I.G.
7. A Universal Method for Labeling Native RNA in Live Bacterial Cells
Irina Smolina and Natalia Broude
8. Live Cell Imaging Using Riboswitch-Spinach tRNA Fusions as Metabolite-Sensing Fluorescent Biosensors
Colleen A. Kellenberger, Zachary F. Hallberg, and Ming C. Hammond
9. RNA Scaffold: Designed To Co-Localize Enzymes
ZJU_China team and Ming Chen
10. Artificial Ligase Ribozymes Isolated by a “Design And Selection” Strategy
Shigeyoshi Matsumura and Yoshiya Ikawa
11. Engineering Aptazyme Switches for Conditional Gene Expression in Mammalian Cells Utilizing an In Vivo Screening Approach
Charlotte Rehm, Benedikt Klauser, and Jörg S. Hartig
12. Aptazyme-Based Riboswitches and Logic Gates in Mammalian Cells
Yoko Nomura and Yohei Yokobayashi
13. Design and Characterisation of Topological Small RNAs
Jack Hassall, Paul MacDonald, Teresa Cordero, William Rostain, and Alfonso Jaramillo
14. Folding RNA-Protein Complex into Designed Nanostructures
Tomonori Shibata, Yuki Suzuki, Hiroshi Sugiyama, Masayuki Endo, and Hirohide Saito
15. Simple Method for Constructing RNA Triangle, Square, Pentagon by Tuning Interior RNA 3WJ Angle from 60˚ to 90˚ or 108˚
Emil F. Khisamutdinov, My Nguyen Hoan Bui, Daniel Jasinski, Zhengyi Zhao, Zheng Cui, and Peixuan Guo
16. RNA-Mediated CdS Based Nanostructures
Vinit Kumar and Anil Kumar
17. An Effective Method for Specific Gene Silencing in Escherichia coli using Artificial Small RNA
Geunu Bak, Jee Soo Choi, Wonkyeong Kim, Shinae Suk, and Younghoon Lee
