Animal Endo-SiRNAs Methods and Protocols

Targeted Small Non-coding RNA Directed Gene Activation in Human Cells.- Isolation of Chromatoid Dodies from Mouse Testis as a Rich Source of Short RNAs.- Generation of Endo-siRNAs in
Xenopus laevis
Oocytes.- Analysis of Endo-siRNAs in Drosophila.- Methods for Studying the Biological Consequences of Endo-siRNA Deficiency in
Drosophila melanogaster
.-
Small RNA Library Cloning Procedure for Deep Sequencing of Specific Endogenous siRNA Classes in
Caenorhabditis elegans
.-
Assays for Direct and Indirect Effects of
C. elegans
Endo-siRNAs.- Extraction and Non-radioactive Detection of Small RNA Molecules.- p19 Mediated Enrichment and Detection of siRNAs.- Detection of Small non-coding RNAs by
in situ
Hybridization using Probes of 2’-O-Methyl RNA + LNA.- Enhanced Detection of Small RNAs using a non-Radioactive Approach.- Computing siRNA and piRNA Overlap Signatures.- Isolation of Small Interfering RNAs using Viral Suppressors of RNA Interference.- Computational Analysis, Biochemical Purification and Detection of tRNA-derived Small RNA Fragments.- Differential DNA Methylation Patterns in Endo-siRNAs Mediated Silencing of LINE-1 Retrotransposons.