Produktbild: Kidney Development
Band 886

Kidney Development Methods and Protocols

Fr. 188.00

inkl. gesetzl. MwSt., Versandkostenfrei


Beschreibung

Produktdetails

Einband

Taschenbuch

Erscheinungsdatum

23.08.2016

Herausgeber

Odyssé Michos

Verlag

Humana Press

Seitenzahl

415

Maße (L/B/H)

25.4/17.8/2.4 cm

Gewicht

817 g

Auflage

Softcover reprint of the original 1st ed. 2012

Sprache

Englisch

ISBN

978-1-4939-6255-6

Beschreibung

Produktdetails

Einband

Taschenbuch

Erscheinungsdatum

23.08.2016

Herausgeber

Odyssé Michos

Verlag

Humana Press

Seitenzahl

415

Maße (L/B/H)

25.4/17.8/2.4 cm

Gewicht

817 g

Auflage

Softcover reprint of the original 1st ed. 2012

Sprache

Englisch

ISBN

978-1-4939-6255-6

Herstelleradresse

Springer-Verlag GmbH
Tiergartenstr. 17
69121 Heidelberg
DE

Email: ProductSafety@springernature.com

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  • Produktbild: Kidney Development
  • Part I: Organ Culture, Tissue Manipulation, and Live Imaging 1. Dissecting and Culturing and Imaging the Mouse Urogenital SystemPaul N. Riccio and Odyssé Michos 2. In Vitro Culture of Embryonic Kidney Rudiments and Isolated Ureteric BudsXing Zhang, Kevin T. Bush and Sanjay K. Nigam 3. In vitro Induction of Nephrogenesis in Mouse Metanephric Mesenchyme with Lithium Introduction and Ureteric Bud RecombinationKimmo Halt and Seppo Vainio 4. Live Imaging of the Developing Mouse MesonephrosDavid Grote, Michael Marcotte, and Maxime Bouchard5. Organotypic Culture of the Urogenital TractEkaterina Batourina, Devangini Gandhi, Cathy L. Mendelsohn and Andrei Molotkov 6. Live Imaging Kidney Development in ZebrafishAleksandr Vasilyev and Iain A. Drummond Part II: Analyzing Ureteric Bud Branching and Nephrogenesis  7. Analysis of 3D Branching Pattern: Hematoxylin and Eosin MethodSunder Sims-Lucas 8. Three-Dimensional Imaging of Fetal Mouse KidneysDeborah Hyink9. Analysis of Native Kidney Structures in Three DimensionsKieran M. Short and Ian M. Smyth 10. Estimating Nephron Number in the Developing Kidney using the Physical Disector/Fractionator CombinationLuise A. Cullen-McEwen, James A. Armitage, Jens R. Nyengaard and John F. Bertram 11. An Immunofluorescence Method to Analyze the Proliferation Status of Individual Nephron Segments in the Xenopus Pronephric KidneyDaniel Romaker, Bo Zhang and Oliver Wessely Part III: Renal Primary Cell Cultures and Cell Lines 12. Dissociation of Embryonic Kidney Followed by Re-aggregation as a Method for Chimeric Analysis Jamie Davies, Mathieu Unbekandt, Jess Ineson, Michael Lusis and Melissa Little 13. Analysis of Migration in Primary Ureteric Bud Epithelial CellsSatu Kuure 14. Investigating Primary Cilia in Cultured Metanephric Mesenchymal CellsLijun Chi and Norman Rosenblum 15. Making Immortalized Cell Lines from Embryonic Mouse KidneyGuanping Tai, Peter Hohenstein and Jamie Davies 16. Engineered Tissues to Quantify Collective Cell Migration During MorphogenesisSriram Manivannan, Jason P. Gleghorn and Celeste M. Nelson Part IV: Detecting Gene/Protein Expression and Signaling 17. Access and use of the GUDMAP Database of Genitourinary DevelopmentJamie A. Davies, Melissa H. Little, Bruce Aronow, Jane Armstrong, Jane Brennan, Sue Lloyd-MacGilp, Chris Armit, Simon Harding, Xinjun Piu, Yogmatee Roochun, Bernard Haggarty, Derek Houghton, Duncan Davidson and Richard Baldock 18. Isolation of High Quality RNA from Embryonic Kidney and CellsShifaan Thowfeequ and Odyssé Michos 19. Laser CaptureSteven S. Potter and Eric W. Brunskill 20. Use of in Situ Hybridization to Examine Gene Expression in the Embryonic, Neonatal and Adult Urogenital SystemBree A. Rumballe, Han Sheng Chiu, Kylie M. Georgas and Melissa H. Little 21. Detection of β-galactosidase Activity: X-gal StainingSally F. Burn 22. Fluorescent Immunolabeling of Embryonic Kidney SamplesCristina Cebrián  23. Immunohistochemical Staining of dpERK Staining During Early Kidney Development Xuan Chi and Odyssé Michos 24. Sensing BMP Pathway Activity by Immune Detection of Phosphorylated R-Smad Proteins in Mouse Embryonic KidneyJavier Lopez-Rios  25. Analysis of in vivo Transcription Factor Recruitment by Chromatin Immunoprecipitation (ChIP) of Mouse Embryonic KidneyClaire Heliot and Silvia CereghiniPart V: Manipulating Gene Expression in Developing and Adult Kidney 26. siRNA-mediated RNA Interference in Embryonic Kidney Organ CultureJamie A. Davies and Mathieu Unbekandt 27. Morpholino-mediated Gene Knockdown in Mammalian Organ CultureAlda Tufro 28. Microinjection into the Lumen of the Ureteric Tree Cristina Cebrián  29. Renal Delivery of Adenovirus and Antisense Oligonucleotides in Rats by Retrograde Renal Vein InjectionGuadalupe Ortiz-Muñoz, Beñat Mallavia, Oscar Lopez-Franco, Purificacion Hernandez-Vargas, Jesus Egido and Carmen Gomez-Guerrero Part VI: Analyzing Functional Defects of the Kidney and Urinary Tract 30. Estimating Total Nephron Number in the Adult Kidney using the Physical Dissector/Fractionator CombinationLuise A. Cullen-McEwen, Rebecca N. Douglas-Denton and John F Bertram 31. Assessing Urinary Tract Defects in Mice: Methods to Detect the Presence of Vesico-ureteric Reflux and Urinary Tract ObstructionInga J. Murawski Christine L. Watt and Indra R. Gupta 32. Ischemia-reperfusion Injury of the Mouse KidneyLeif Oxburgh and Mark P. de Caestecker  33. Variable Partial Unilateral Ureteral Obstruction and its Release in the Neonatal and Adult MouseBarbara A. Thornhill and Robert L. Chevalier 34. Urinary Diversion Via Cutaneous Vesicostomy in the Megabladder MouseAshley R. Carpenter, Brian Becknell, Daniel A. Hirselj and Kirk M. McHugh 35. Ultrasound Imaging of the Murine KidneyAshley Carpenter, Brian Becknell, Susan E. Ingraham and Kirk M. McHugh