Membrane Protein Structure and Dynamics Methods and Protocols

Crystallization of Membrane Proteins in Bicelles.-Vapor Diffusion Controlled
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Crystallization of Membrane Proteins.-Solution NMR Studies of Integral Polytopic -helical Membrane Proteins: The Structure Determination of the Seven-helix Transmembrane Receptor Sensory Rhodopsin II, pSRII.-Use of NMR Saturation Transfer Difference Spectroscopy to Study Ligand Binding to Membrane Proteins.-How to Investigate Interactions Between Membrane Proteins and Ligands by Solid-state NMR.-Identifying and Measuring Transmembrane Helix-helix Interactions by FRET.-Studying Substrate Binding to Reconstituted Secondary Transporters by Attenuated Total Reflection Infrared Difference Spectroscopy.-UV-Visible and Infrared Methods for Investigating Lipid-Rhodopsin Membrane Interactions.-Proteomic Characterization of Integral Membrane Proteins Using Thermostatted Liquid Chromatography Coupled with Tandem Mass Spectrometry.-LITiCon: A Discrete Conformational Sampling Computational Method for Mapping Various Functionally Selective Conformational States of Transmembrane Helical Proteins.-Homology Model-assisted Elucidation of Binding Sites in GPCRs.-Comparative Modeling of Lipid Receptors.-Quantification of Structural Distortions in the Transmembrane Helices of GPCRs.-Structure Prediction of G Protein-Coupled Receptors and Their Ensemble of Functionally Important Conformations.-Target Based Virtual Screening by Docking into Automatically Generated GPCR Models.-Predicting the Biological Activities through QSAR Analysis and Docking-based Scoring.-Identification of Motions in Membrane Proteins by Elastic Network Models and Their Experimental Validation.-Modeling theStructural Communication in Supramolecular Complexes Involving GPCRs.-Exploring Substrate Diffusion in Channels using Biased Molecular Dynamics Simulations.