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Produktbild: Handbook of ELISPOT
Band 792

Handbook of ELISPOT Methods and Protocols

Aus der Reihe Methods in Molecular Biology

Fr. 150.00

inkl. gesetzl. MwSt., Versandkostenfrei

Beschreibung

Produktdetails

Einband

Taschenbuch

Erscheinungsdatum

23.08.2016

Abbildungen

X, 59 illus., 13 illus. in color., schwarz-weiss Illustrationen, farbige Illustrationen

Herausgeber

Alexander E. Kalyuzhny

Verlag

Humana Press

Seitenzahl

261

Maße (L/B/H)

25.4/17.8/1.5 cm

Gewicht

522 g

Auflage

2. Auflage

Sprache

Englisch

ISBN

978-1-4939-6111-5

Beschreibung

Rezension

From the reviews of the second edition:

“In this second edition … the reader is introduced not only to the basic concept behind the technique but also to many different applications in common use. Importantly, the book serves as a reference guide with lots of trouble shooting and discussion of common problems. … This text would be key reading material for any laboratory planning to set up this technique. … this text is comprehensive and detailed with a good use of raw data and formula to make following the protocols simple.” (Hayley Evans, Immunology News, November, 2012)

Produktdetails

Einband

Taschenbuch

Erscheinungsdatum

23.08.2016

Abbildungen

X, 59 illus., 13 illus. in color., schwarz-weiss Illustrationen, farbige Illustrationen

Herausgeber

Alexander E. Kalyuzhny

Verlag

Humana Press

Seitenzahl

261

Maße (L/B/H)

25.4/17.8/1.5 cm

Gewicht

522 g

Auflage

2. Auflage

Sprache

Englisch

ISBN

978-1-4939-6111-5

Herstelleradresse

Springer-Verlag GmbH
Tiergartenstr. 17
69121 Heidelberg
DE

Email: ProductSafety@springernature.com

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  • Produktbild: Handbook of ELISPOT
  • Part I: Setting and Performing ELISPOT Assay 1. Unique Strengths of ELISPOT for T Cell Diagnostics            Paul V. Lehmann and Wenji Zhang 2. The Impact of Harmonization on ELISPOT Assay Performance 7. ELISPOT Assay as a Tool to Study Oxidative Stress in Lymphocytes            Jodi Hagen, Jeffrey P. Houchins, and Alexander E. Kalyuzhny 8. ELISPOT Assay for Neuroscience Research: Studying TNFa Secretion from Microglial Cells            Jodi Hagen, Jeffrey P. Houchins, and Alexander E. Kalyuzhny 9. ELISPOT Assay as a Tool to Study the Effects of Stem Cells on Cytokine Secretion            Jun-Seop Shin and Chung-Gyu Park 10. Combining ELISPOT and ELISA to Measure Amounts of Cytokines Secreted by a Single Cell            Jodi Hagen, Jeffrey P. Houchins, and Alexander E. Kalyuzhny Part IV. Image and Data Analysis11. How ELISPOT Morphology Reflects on the Productivity and Kinetics of Cells’ Secretory Activity            Sylvia Janetzki and Cedrik M. Britten Part II: ELISPOT for Veterinary Research 3. Equine ELISPOT Assay to Study Secretion of IFNγ and IL-4 from Peripheral Blood Mononuclear Cells 7. ELISPOT Assay as a Tool to Study Oxidative Stress in Lymphocytes            Jodi Hagen, Jeffrey P. Houchins, and Alexander E. Kalyuzhny 8. ELISPOT Assay for Neuroscience Research: Studying TNFa Secretion from Microglial Cells            Jodi Hagen, Jeffrey P. Houchins, and Alexander E. Kalyuzhny 9. ELISPOT Assay as a Tool to Study the Effects of Stem Cells on Cytokine Secretion            Jun-Seop Shin and Chung-Gyu Park 10. Combining ELISPOT and ELISA to Measure Amounts of Cytokines Secreted by a Single Cell            Jodi Hagen, Jeffrey P. Houchins, and Alexander E. Kalyuzhny Part IV. Image and Data Analysis11. How ELISPOT Morphology Reflects on the Productivity and Kinetics of Cells’ Secretory Activity            Jodi Hagen, Chris Hartnett, J.P. Houchins, Steeve Giguère, and Alexander E. Kalyuzhny 4. Utilization of Feline ELISPOT for Mapping Vaccine Epitopes 7. ELISPOT Assay as a Tool to Study Oxidative Stress in Lymphocytes            Jodi Hagen, Jeffrey P. Houchins, and Alexander E. Kalyuzhny 8. ELISPOT Assay for Neuroscience Research: Studying TNFa Secretion from Microglial Cells            Jodi Hagen, Jeffrey P. Houchins, and Alexander E. Kalyuzhny 9. ELISPOT Assay as a Tool to Study the Effects of Stem Cells on Cytokine Secretion            Jun-Seop Shin and Chung-Gyu Park 10. Combining ELISPOT and ELISA to Measure Amounts of Cytokines Secreted by a Single Cell            Jodi Hagen, Jeffrey P. Houchins, and Alexander E. Kalyuzhny Part IV. Image and Data Analysis11. How ELISPOT Morphology Reflects on the Productivity and Kinetics of Cells’ Secretory Activity            Jeffrey R. Abbott, Ruiyu Pu, James K. Coleman, and Janet K. Yamamoto 5. Analyzing Cellular Immunity to AAV in a Canine Model Using ELISPOT Assay 7. ELISPOT Assay as a Tool to Study Oxidative Stress in Lymphocytes            Jodi Hagen, Jeffrey P. Houchins, and Alexander E. Kalyuzhny 8. ELISPOT Assay for Neuroscience Research: Studying TNFa Secretion from Microglial Cells            Jodi Hagen, Jeffrey P. Houchins, and Alexander E. Kalyuzhny 9. ELISPOT Assay as a Tool to Study the Effects of Stem Cells on Cytokine Secretion            Jun-Seop Shin and Chung-Gyu Park 10. Combining ELISPOT and ELISA to Measure Amounts of Cytokines Secreted by a Single Cell            Jodi Hagen, Jeffrey P. Houchins, and Alexander E. Kalyuzhny Part IV. Image and Data Analysis11. How ELISPOT Morphology Reflects on the Productivity and Kinetics of Cells’ Secretory Activity            Zejing Wang, Rainer Storb, Stephen J. Tapscott, and Stanley Riddell Part III: Advanced ELISPOT Techniques 6. Dual and Triple Color Fluorospot 7. ELISPOT Assay as a Tool to Study Oxidative Stress in Lymphocytes            Jodi Hagen, Jeffrey P. Houchins, and Alexander E. Kalyuzhny 8. ELISPOT Assay for Neuroscience Research: Studying TNFa Secretion from Microglial Cells            Jodi Hagen, Jeffrey P. Houchins, and Alexander E. Kalyuzhny 9. ELISPOT Assay as a Tool to Study the Effects of Stem Cells on Cytokine Secretion            Jun-Seop Shin and Chung-Gyu Park 10. Combining ELISPOT and ELISA to Measure Amounts of Cytokines Secreted by a Single Cell            Jodi Hagen, Jeffrey P. Houchins, and Alexander E. Kalyuzhny Part IV. Image and Data Analysis11. How ELISPOT Morphology Reflects on the Productivity and Kinetics of Cells’ Secretory Activity            Niklas Ahlborg and Bernt Axelsson            Alexey Y. Karulin and Paul V. Lehmann 12. Mathematical Algorithms for Automatic Search, Recognition, and Detection of Spots in ELISPOT Assay            Sergey S. Zadorozhny and Nikolai N. Martynov 13. Objective, User-Independent ELISPOT Data Analysis Based on Scientifically Validated Principles            Wenji Zhang and Paul V. Lehmann 14. Statistical Analysis of ELISPOT Assays            Marcus Dittrich and Paul V. Lehmann 15. Response Determination Criteria for ELISPOT: Toward a Standard that Can Be Applied Across Laboratories            Zoe Moodie, Leah Price, Sylvia Janetzki, and Cedrik M. Britten Part V: ELISPOT Assay for Vaccine Development and Diagnostics 16. Detection of Vaccinia Virus-Specific IFNγ and IL-10 Secretion from Human PBMCs and CD8+ T Cells by ELISPOT            Benjamin J. Umlauf, Norman A. Pinsky, Inna G. Ovsyannikova, and Gregory A. Poland 17. ELISPOT Assays to Enumerate Bovine IFN-γ Secreting Cells for the Development of Novel Vaccines Against Bovine Tuberculosis            Martin Vordermeier and Adam O. Whelan 18. IL-7 Addition Increases Spot Size and Number as Measured by T-SPOT.TB®            Marsha L. Feske, Miguel Medina, Edward A. Graviss, and Dorothy E. Lewis 19. Overview of Membranes and Membrane Plates Used in Research and Diagnostic ELISPOT Assays            Alan J. Weiss 7. ELISPOT Assay as a Tool to Study Oxidative Stress in Lymphocytes            Jodi Hagen, Jeffrey P. Houchins, and Alexander E. Kalyuzhny 8. ELISPOT Assay for Neuroscience Research: Studying TNFa Secretion from Microglial Cells            Jodi Hagen, Jeffrey P. Houchins, and Alexander E. Kalyuzhny 9. ELISPOT Assay as a Tool to Study the Effects of Stem Cells on Cytokine Secretion            Jun-Seop Shin and Chung-Gyu Park 10. Combining ELISPOT and ELISA to Measure Amounts of Cytokines Secreted by a Single Cell            Jodi Hagen, Jeffrey P. Houchins, and Alexander E. Kalyuzhny Part IV. Image and Data Analysis11. How ELISPOT Morphology Reflects on the Productivity and Kinetics of Cells’ Secretory Activity            Alexey Y. Karulin and Paul V. Lehmann 12. Mathematical Algorithms for Automatic Search, Recognition, and Detection of Spots in ELISPOT Assay            Sergey S. Zadorozhny and Nikolai N. Martynov 13. Objective, User-Independent ELISPOT Data Analysis Based on Scientifically Validated Principles            Wenji Zhang and Paul V. Lehmann 14. Statistical Analysis of ELISPOT Assays            Marcus Dittrich and Paul V. Lehmann 15. Response Determination Criteria for ELISPOT: Toward a Standard that Can Be Applied Across Laboratories            Zoe Moodie, Leah Price, Sylvia Janetzki, and Cedrik M. Britten Part V: ELISPOT Assay for Vaccine Development and Diagnostics 16. Detection of Vaccinia Virus-Specific IFNγ and IL-10 Secretion from Human PBMCs and CD8+ T Cells by ELISPOT            Benjamin J. Umlauf, Norman A. Pinsky, Inna G. Ovsyannikova, and Gregory A. Poland 17. ELISPOT Assays to Enumerate Bovine IFN-γ Secreting Cells for the Development of Novel Vaccines Against Bovine Tuberculosis            Martin Vordermeier and Adam O. Whelan 18. IL-7 Addition Increases Spot Size and Number as Measured by T-SPOT.TB®            Marsha L. Feske, Miguel Medina, Edward A. Graviss, and Dorothy E. Lewis 19. Overview of Membranes and Membrane Plates Used in Research and Diagnostic ELISPOT Assays            Alan J. Weiss