Preface…
Table of Contents…
Contributing Authors…
Part I Introduction
1. Vitamin B3 in Health and Disease: Toward the Second Century of Discovery
Myron K. Jacobson and Elaine L. Jacobson
Part II ADPribose Binding Domains as Tools
2. Monitoring Poly(ADP-Ribosyl)ation in Response to DNA Damage in Live Cells Using Fluorescently-Tagged Macrodomains
Rebecca Smith and Gyula Timinszky
3. In Vitro Techniques for ADP-Ribosylated Substrate Identification
Giovanna Grimaldi, Giulianan Catara, Carmen Valente, and Daniela Corda
4. Assessment of Intracellular Auto-Modification Levels of ARTD10 using Mono-ADP-Ribose-Specific Macrodomains 2 and 3 of Murine Artd8
Mareike Bütepage, Sarah Krieg, laura Eckei, Jinyu Li, Giulia Rossetti, Patricia Verheugd, and Bernhard Lüscher
5. Biochemical and Biophysical Assays of PAR-WWE Domain Interactions and Production of iso-ADPr for PAR Binding Analysis
Zhizhi Wang and Wenqing Xu
Part III Enzymatic Assays of NAD+ Utilizing Enzymes
6. Assays for NAD-Dependent Reactions and NAD Metabolites
Michael B. Schultz, Yuancheng Lu, Nady Braidy, and David A Sinclair
7. Generating Protein-Linked and Protein-Free Mono-, Oligo-, and Poly(ADP-Ribose) In Vitro
Ken Y. Lin, Dan Huang, and W. Lee Kraus
8. Methods to Study TCDD-Inducible Poly-ADP-Ribose Polymerase (TIPARP) Mono-ADP-Ribosyltransferase Activity
David Hutin, Giulia Grimaldi, and Jason Matthews
9. Dictyostelium as a Model to Assess Site-Specific ADP-Ribosylation Events
Anna-Lena Kolb, Duen-Wei Hsu, Ana B.A. Wallis, Seiji Ura, Alina Rakhimova, Catherine J. Pears, and Nicholas D. Lakin
10. Mono-ADP-Ribosylation Catalyzed by Arginine-Specific ADP-Ribosyltransferases
Linda A. Stevens and Joel Moss
11. Monitoring Expression and Enzyme Activity of Ecto-ARTCs
Stephan Menzel, Sahil Abriouch, Peter Bannas, Friedrich Haag, and Friedrich Koch-Nolte
12. ADP-Ribosyl-Acceptor Hydrolase Activities Catalyzed by the ARH Family of Proteins
Masato Mashimo and Joel Moss
13. Mono-ADP-Ribosylhydrolase Assays
Jeannette Abplanalp, Ann-Katrin Hopp, and Michael O. Hottiger
14. Hydrolysis of ADP-Ribosylation by Macrodomains
Melanija Posavec Marjanović, Gytis Jankevicius, and Ivan Ahel
15. HPLC-Based Enzyme Assays for Sirtuins
Jun Young Hong, Xiaoyu Zhang, and Hening Lin
Part IV Small Molecule Screening Assays of NAD+ Utilizing Enzymes
16. Small Molecule Screening Assay for Mono-ADP-Ribosyltransferases
Haikarainen Teemu, Murphy Sudarshan, Maksimainen Mirko, and Lari Lehtiö
17. ¬A Simple, Sensitive, and Generalizable Plate Assay for Screening PARP Inhibitors
Ilsa T. Kirby, Rory K. Morgan, and Michael S. Cohen
Part V Mass Spectrometry Techniques for Detection of Mono-ADP-Ribosylation
18. Non-Localized Searching of HCD Data for Fast and Sensitive Identification of ADP-Ribosylated Peptides
Thomas Colby, Juan José Bonfiglio, and Ivan Matic
19. Quantitative Determination of MAR Hydrolase Residue Specificity In Vitro by Tandem Mass Spectrometry
Robert Lyle Mcpherson, Shao-En Ong, and Anthony K.L. Leung
Part VI Mono-ADP-Ribose and Disease
20. Detection of ADP-Ribosylating Bacterial Toxins
Chen Chen and Joseph T. Barbieri
21. Preparation of Recombinant Alphaviruses for Functional Studies of ADP-Ribosylation
Rachy Abraham, Robert L. McPherson, Easwaran Sreekumar, Anthony K.L. Leung, and Diane E. Griffin
22. Monitoring the Sensitivity of T Cell Populations towards NAD+ Released during Cell Preparation
Björn Rissiek, Marco Lukowiak, Friedrich Haag, Tim Magnus, and Friedrich Koch-Nolte
23. Identifying Target RNAs of PARPs
Florian J. Bock and Paul Chang
Part VII NAD+ Analogue Approaches and Chemical Synthesis
24. ADPr-Peptide Synthesis
Hans A.V. Kistemaker, Jim Voorneveld, and Dmitri V. Filippov
25. Identifying Genomic Sites of ADP-Ribosylation Mediated by Specific Nuclear PARP Enzymes Using Click-ChIP
Ryan A. Rogge, Bryan A. Gibson, and W. Lee Kraus
Part VIII NAD+ Sensors
26. Methods for Using a Genetically-Encoded Fluorescent Biosensor to Monitor Nuclear NAD+
Michael S. Cohen, Melissa L. Stewart, Richard H. Goodman, and Xiaolu A. Cambronne
