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Archaea Methods and Protocols

Aus der Reihe Methods in Molecular Biology

Fr. 149.00

inkl. gesetzl. MwSt., Versandkostenfrei

Beschreibung

Produktdetails

Einband

Taschenbuch

Erscheinungsdatum

22.09.2023

Herausgeber

Sébastien Ferreira-Cerca

Verlag

Springer Us

Seitenzahl

549

Maße (L/B/H)

25.4/17.8/3.1 cm

Gewicht

1047 g

Auflage

1st ed. 2022

Sprache

Englisch

ISBN

978-1-07-162447-0

Beschreibung

Produktdetails

Einband

Taschenbuch

Erscheinungsdatum

22.09.2023

Herausgeber

Sébastien Ferreira-Cerca

Verlag

Springer Us

Seitenzahl

549

Maße (L/B/H)

25.4/17.8/3.1 cm

Gewicht

1047 g

Auflage

1st ed. 2022

Sprache

Englisch

ISBN

978-1-07-162447-0

Herstelleradresse

Libri GmbH
Europaallee 1
36244 Bad Hersfeld
DE

Email: GPSR Kontakt

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  • Produktbild: Archaea
  • Produktbild: Archaea
  • Archaea: a goldmine for molecular biologists and evolutionists.- Progress and challenges in archaeal genetic manipulation.- Genetic manipulation of Haloferax species.- CRISPR interference as a tool to Repress Gene Expression in Haloferax volcanii.-  Transformation Techniques for the Anaerobic Hyperthermophile Thermococcus kodakarensis.-  Genetic Methods and Construction of Chromosomal Mutations in Methanogenic Archaea.- An interdomain conjugation protocol for plasmid-DNA transfer into Methanothermobacter thermautotrophicus ΔH.- Methods for Markerless Gene Deletion and Plasmid-Based Expression in Sulfolobus acidocaldarius.-  A Rapid Targeted Gene Inactivation Approach in Sulfolobus islandicus.-  Transposon Insertion Mutagenesis in hyperthermophilic crenarchaeon Sulfolobus islandicus.-  Reprogramming CRISPR-mediated RNA interference for silencing of essential genes in Sulfolobales.- Progress and challenges in archaeal molecular biology.- ChIP-seq Occupancy Mapping of the Archaeal Transcription Machinery.- Conversion of ribosome-protected mRNA to DNA and deep sequencing for ribosome profiling in Haloferax volcanii.-  Small RNA-sequencing library preparation for the halophilic archaeon Haloferax volcanii.-  Quantitative Mass Spectrometry by SILAC in Haloferax volcanii.-  Proteome turnover analysis in Haloferax volcanii by a heavy isotope multilabelling approach.- Proteomic sample preparation and data analysis in line with the Archaeal Proteome.- In vivo protein crosslinking and co-immunoprecipitation in Haloferax volcanii.-  Proteolytic activity assays in haloarchaea.- Carotenoids from Haloarchaea: Extraction, fractionation and characterization.- Metal nanoparticles biosynthesis using the halophilic archaeon Haloferax volcanii.-  Simplified enzymatic synthesis of 2-keto-3-deoxy-D-gluconate from D-gluconate using the gluconate dehydratase from Thermoproteus tenax.-  Progress and Challenges in Archaeal Cellular Biology Analysis.- Methods to Analyze Motility in Eury- And Crenarchaea.- Immersed Liquid Biofilm and Honeycomb Pattern Formations in Haloferax volcanii.-  Cost-effective and Versatile Analysis of Archaeal Surface Adhesion Under Shaking and Standing Conditions.- Cell Adhesion and Biofilm Formation Analysis.- BrdU incorporation and labelling of nascent DNA to Investigate Archaeal Replication using super-resolution imaging.- Isolation, Purification, and Characterization of Membrane Vesicles from Haloarchaea.- Archaeal Viruses: Production of Virus Particles and Vesicle-Like Viruses and Purification Using Asymmetrical Flow Field-Flow Fractionation.- Progress and Challenges in Studying the Ecophysiology of Archaea.- Reconstruction of Archaeal Genomes Assembly from Short-Read Metagenomes.- Outreach: Accessible and Insightful Scientific Learning Experiences Using the Microorganism Haloferax volcanii.