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Produktbild: Macrophage Migration Inhibitory Factor
Band 3002

Macrophage Migration Inhibitory Factor Methods and Protocols

Aus der Reihe Methods in Molecular Biology

Fr. 241.00

inkl. gesetzl. MwSt., Versandkostenfrei

Beschreibung

Produktdetails

Einband

Gebundene Ausgabe

Erscheinungsdatum

15.05.2026

Abbildungen

XII, 296 p. 93 illus., 83 illus. in color.

Herausgeber

James Harris + weitere

Verlag

Springer Us

Seitenzahl

296

Maße (L/B/H)

26/18.3/2.3 cm

Gewicht

778 g

Auflage

Second Edition 2026

Sprache

Englisch

ISBN

978-1-07-165055-4

Beschreibung

Produktdetails

Einband

Gebundene Ausgabe

Erscheinungsdatum

15.05.2026

Abbildungen

XII, 296 p. 93 illus., 83 illus. in color.

Herausgeber

Verlag

Springer Us

Seitenzahl

296

Maße (L/B/H)

26/18.3/2.3 cm

Gewicht

778 g

Auflage

Second Edition 2026

Sprache

Englisch

ISBN

978-1-07-165055-4

Herstelleradresse

Libri GmbH
Europaallee 1
36244 Bad Hersfeld
DE

Email: gpsr@libri.de

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  • Produktbild: Macrophage Migration Inhibitory Factor

  • Expression and Purification of Recombinant Macrophage Migration Inhibitory Factor (MIF).- Generation of MIF Peptides via Solid Phase Peptide Synthesis.- Expression and Purification of Recombinant D-Dopachrome Tautomerase (D-DT).- Structural Determination and Ligand Interaction of Macrophage Inhibitory Factor: A Standardized X-Ray Crystallography Approach.- Generation of High-Quality D-Dopachrome Tautomerase Crystals for Structural Studies.- Fragment Screening of MIF by Surface Plasmon Resonance.- Detection of Noncovalent Protein-Ligand Complexes of D-DT Variants by MALDI-TOF Mass Spectrometry.- Analysis of D-Dopachrome Tautomerase Conformational Flexibility Through Molecular Dynamics Solutions.- MIF NMR Chemical Shift Perturbation Mapping.- Assessing the Tautomerase Activity of MIF Using L-Dopachrome.- Using 4-HPP and PP as Substrates to Assess Purified MIF and D-DT Tautomerase Activity and Inhibition.- Protocols for Assessing the Nuclease Activity of MIF.- Detection of Oxidized Macrophage Migration Inhibitory Factor (oxMIF) in Biological Samples Using Enzyme-Linked Immunosorbent Assay (ELISA).- Detection of Oxidized Macrophage Migration Inhibitory Factor (oxMIF) in Tissue Samples Using Immunohistochemistry (IHC).- Exploring the Chemotactic Effects of MIF on Neutrophils and T Cells and Methods for Selective Receptor Targeting.- Polarization of Bone Marrow-Derived Macrophages from Wild-Type and High Human MIF-Expressing CATT
    7
     Polymorphic Mice.- Using Luciferase Reporter Cells to Assess the Effects of MIF Inhibitors on NF-kB Activation.- Monitoring Inflammasome Activity through ASC Speck Formation in THP-1 ASC-GFP Cells.- Small Interfering RNA (siRNA)-Mediated Knockdown of Macrophage Migration Inhibitory Factor Transcript in Adherent and Suspension Cultures of Human Cell Lines.- Stable Transfection of
    CD74
    Gene Variants into A549 Lung Adenocarcinoma Cell Line.- Methods to Determine the Role of MIF in Cilia Biogenesis.- Modelling Fungal and Amoebic Infections: Co-Culture Approaches to Study MIF’s Role in Infection Induced Inflammatory Responses.- Immunofluorescent Quantification of MIF in Larval Zebrafish Tailfin Transfection.