Genetic Transformation Systems in Fungi, Volume 2

I. Endogenous DNA: Cell Fusion

1. Anastomosis and Heterokaryon Formation

Martin Weichert and André Fleissner

2. Induction of the Sexual Cycle in Filamentous Ascomycetes

Jos Houbraken and Paul S. Dyer

3. What Have We Learned by Doing Transformations in Neurospora tetrasperma?

Durgadas P. Kasbekar

II. Endogenous DNA: Repetitive Elements

4. Repeat-induced Point Mutation: A Fungal-specific, Endogenous Mutagenesis Process

James K. Hane, Angela H. Williams, Adam P. Taranto, Peter S. Solomon and Richard P. Oliver

5. Calculating RIP Mutation in Fungal Genomes Using RIPCAL

James K. Hane

6. Fungal Transposable Elements

Linda Paun and Frank Kempken

7. In vivo Targeted Mutagenesis in Yeast using TaGTEAM

Shawn Finney-Manchester and Narendra Maheshri

III. Endogenous DNA: RNAi

8. RNA Silencing in Filamentous Fungi: from Basic to Applications

Nguyen Bao Quoc and Hitoshi Nakayashiki

9. RNAi-mediated Gene Silencing in the Beta-lactam Producers Fungi Penicillium chrysogenum and Acremonium chrysogenum

Carlos García-Estrada and Ricardo V. Ullán

10. Controlling Fungal Gene Expression Using the Doxycycline-Dependent Tet-ON System in Aspergillus fumigatus

Michaela Dümig and Sven Krappmann

IV. Tools & Applications: Selection Markers and Vectors

11. Expanding the Repertoire of Selectable Markers for Aspergillus Transformation

Khyati Dave, V. Lakshmi Prabha, Manmeet Ahuja, Kashyap Dave, S. Tejaswini and Narayan S. Punekar

12. Arginase (agaA) as a Fungal Transformation Marker

Kashyap Dave, Manmeet Ahuja, Jayashri, T.N., Rekha Bisht Sirola, Khyati Dave and Narayan S. Punekar

13. Transformation of Ascomycetous Fungi using Autonomously Replicating Vectors

Takeo Shimizu and Satoko Kanematsu

14. A Recyclable and Bidirectionally Selectable Marker System for Transformation of Trichoderma

Thiago M. Mello-de-Sousa, Robert L. Mach and Astrid R. Mach-Aigner

15. Split Marker-Mediated Transformation and Targeted Gene Disruption in Filamentous Fungi

Kuang-Ren Chung and Miin-Huey Lee

V. Tools & Applications: High Throughput Experimentation

16. Integrated Automation for Continuous High-Throughput Synthetic Chromosome Assembly and Transformation to Identify Improved Yeast Strains for Industrial Production of Biofuels and Bio-based Chemicals

Stephen R. Hughes and Steven B. Riedmuller

17. Imaging Flow Cytometry and High-throughput Microscopy for Automated Macroscopic Morphological Analysis of Filamentous Fungi

Aydin Golabgir, Daniela Ehgartner, Lukas Neutsch, Andreas E. Posch, Peter Sagmeister and Christoph Herwig

18. Yeast Cell Electroporation in Droplet-Based Microfluidic Chip

Qiuxian Cai and Chunxiong Luo

19. Identification of T-DNA Integration Sites: TAIL-PCR and Sequence Analysis

Jaehyuk Choi, Junhyun Jeon and Yong-Hwan Lee

VI. Tools & Applications: Comprehensive Approaches in Selected Fungi

20. Genetic and Genomic Manipulation in Aspergillus niger

Adrian Tsang, Annie Bellemare, Corinne Darmond and Janny Bakhuis

21. Genetic Manipulation of Meyerozyma guilliermondii

Nicolas Papon,Yuriy R. Boretsky, Vincent Courdavault, Marc Clastre and Andriy A. Sibirny